Woenckhaus Matthias, Bubendorf Lukas, Dalquen Peter, Foerster Julia, Blaszyk Hagen, Mirlacher Martina, Soler Markus, Dietmaier Wolfgang, Sauter Guido, Hartmann Arndt, Wild Peter J
Institute of Pathology, University of Regensburg, Regensburg, Germany.
J Clin Pathol. 2007 May;60(5):483-6. doi: 10.1136/jcp.2005.033407. Epub 2006 May 12.
To investigate whether nuclear and cytoplasmic Maspin expression is associated with distinct clinicopathological parameters and TP53 expression in a representative series of primary non-small cell lung cancer (NSCLC).
Tissue microarrays (n=487) were used to immunohistochemically analyse expression of Maspin and TP53. Cytoplasmic and nuclear expression of Maspin was scored on the basis of the percentage of positive tumour cells. Univariate analysis of clinicopathological variables potentially affecting tumour-specific survival was performed.
Immunohistochemical Maspin expression (nuclear and cytoplasmic) was informative in 72.3% (352/487) of cases. Cytoplasmic and nuclear Maspin immunoreactivity in >or=10% of tumour cells was detected in 37.8% (133/352) and 65.3% (230/352) of informative cases, respectively. Nuclear and cytoplasmic Maspin staining was observed more frequently in primary squamous cell carcinomas than in other lung cancer types. Only nuclear Maspin immunoreactivity was significantly associated with positive TP53 staining. Cytoplasmic or nuclear Maspin expression was not associated with tumour-specific survival.
Maspin expression was found both in the nucleus and the cytoplasm of NSCLC, more frequently in squamous cell carcinomas. However, no association with tumour-specific survival could be demonstrated.
在一组具有代表性的原发性非小细胞肺癌（NSCLC）中，研究细胞核和细胞质中Maspin的表达是否与不同的临床病理参数及TP53表达相关。
使用组织微阵列（n = 487）对Maspin和TP53的表达进行免疫组织化学分析。根据阳性肿瘤细胞的百分比对Maspin的细胞质和细胞核表达进行评分。对可能影响肿瘤特异性生存的临床病理变量进行单因素分析。
免疫组织化学检测显示，72.3%（352/487）的病例中Maspin表达（细胞核和细胞质）具有信息价值。在有信息价值的病例中，分别有37.8%（133/352）和65.3%（230/352）的病例在≥10%的肿瘤细胞中检测到细胞质和细胞核Maspin免疫反应性。与其他肺癌类型相比，原发性鳞状细胞癌中更频繁地观察到细胞核和细胞质Maspin染色。仅细胞核Maspin免疫反应性与TP53染色阳性显著相关。细胞质或细胞核Maspin表达与肿瘤特异性生存无关。
在NSCLC的细胞核和细胞质中均发现了Maspin表达，在鳞状细胞癌中更为常见。然而，未发现其与肿瘤特异性生存相关。
