Bräuer Suzanna L, Cadillo-Quiroz Hinsby, Yashiro Erika, Yavitt Joseph B, Zinder Stephen H
Department of Microbiology, Cornell University, Ithaca, New York 14853, USA
Nature. 2006 Jul 13;442(7099):192-4. doi: 10.1038/nature04810. Epub 2006 May 14.
Acidic peatlands are among the largest natural sources of atmospheric methane and harbour a large diversity of methanogenic Archaea. Despite the ubiquity of methanogens in these peatlands, indigenous methanogens capable of growth at acidic pH values have resisted culture and isolation; these recalcitrant methanogens include members of an uncultured family-level clade in the Methanomicrobiales prevalent in many acidic peat bogs in the Northern Hemisphere. However, we recently succeeded in obtaining a mixed enrichment culture of a member of this clade. Here we describe its isolation and initial characterization. We demonstrate that the optimum pH for methanogenesis by this organism is lower than that of any previously described methanogen.
酸性泥炭地是大气甲烷的最大天然来源之一，并且蕴藏着种类繁多的产甲烷古菌。尽管这些泥炭地中产甲烷菌无处不在，但能够在酸性pH值下生长的本土产甲烷菌一直难以培养和分离；这些顽固的产甲烷菌包括在北半球许多酸性泥炭沼泽中普遍存在的甲烷微菌目中一个未培养的科级分支的成员。然而，我们最近成功获得了该分支一名成员的混合富集培养物。在此，我们描述其分离过程和初步特性。我们证明，该生物体产甲烷的最适pH值低于任何先前描述的产甲烷菌。
