Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):45-52. doi: 10.1099/ijs.0.021782-0. Epub 2010 Feb 12.
A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)).
一株新型嗜酸产氢甲烷菌，命名为 6A8(T) 株，从美国纽约州伊萨卡附近的一处酸性（pH4.0-4.5）和寡营养（雨养）沼泽中分离得到。培养物呈二态性，含有细杆（0.2-0.3μm 直径和 0.8-3.0μm 长）和不规则球菌（0.2-0.8μm 直径）。该培养物利用 H(2)/CO(2) 产生甲烷，但不利用甲酸盐、乙酸盐、甲醇、乙醇、2-丙醇、正丁醇或三甲胺。最佳生长条件为接近 pH5.1 和 35°C。该培养物在含有低至 0.43mM Na(+) 的基础培养基中生长，50mM NaCl 完全抑制其生长。据我们所知，6A8(T) 株是纯培养中最嗜酸（最低 pH 最适值）和最敏感的产甲烷菌之一。乙酸盐、辅酶 M、维生素和酵母提取物是生长所必需的。建议为该生物建立一个新属和新种，命名为 Methanoregula boonei 属，新种。Methanoregula boonei 的模式菌株为 6A8(T)=DSM 21154(T)=JCM 14090(T)。
