Brandolini A, Vaccino P, Boggini G, Ozkan H, Kilian B, Salamini F
Istituto Sperimentale per la Cerealicoltura, CRA, 26866 S. Angelo Lodigiano, Italy.
Genome. 2006 Apr;49(4):297-305. doi: 10.1139/g05-110.
The genetic relationships of A genomes of Triticum urartu (Au) and Triticum monococcum (Am) in polyploid wheats are explored and quantified by AFLP fingerprinting. Forty-one accessions of A-genome diploid wheats, 3 of AG-genome wheats, 19 of AB-genome wheats, 15 of ABD-genome wheats, and 1 of the D-genome donor Ae. tauschii have been analysed. Based on 7 AFLP primer combinations, 423 bands were identified as potentially A genome specific. The bands were reduced to 239 by eliminating those present in autoradiograms of Ae. tauschii, bands interpreted as common to all wheat genomes. Neighbour-joining analysis separates T. urartu from T. monococcum. Triticum urartu has the closest relationship to polyploid wheats. Triticum turgidum subsp. dicoccum and T. turgidum subsp. durum lines are included in tightly linked clusters. The hexaploid spelts occupy positions in the phylogenetic tree intermediate between bread wheats and T. turgidum. The AG-genome accessions cluster in a position quite distant from both diploid and other polyploid wheats. The estimates of similarity between A genomes of diploid and polyploid wheats indicate that, compared with Am, Au has around 20% higher similarity to the genomes of polyploid wheats. Triticum timo pheevii AG genome is molecularly equidistant from those of Au and Am wheats.
通过AFLP指纹图谱技术，对多倍体小麦中乌拉尔图小麦（Au）和一粒小麦（Am）的A基因组之间的遗传关系进行了探索和量化。分析了41份A基因组二倍体小麦材料、3份AG基因组小麦材料、19份AB基因组小麦材料、15份ABD基因组小麦材料以及1份D基因组供体节节麦材料。基于7种AFLP引物组合，共鉴定出423条可能为A基因组特异性的条带。通过去除节节麦放射自显影片中出现的条带（即被认为是所有小麦基因组共有的条带），条带数量减少至239条。邻接法分析将乌拉尔图小麦与一粒小麦区分开来。乌拉尔图小麦与多倍体小麦的关系最为密切。硬粒小麦亚种二粒小麦和硬粒小麦亚种 durum系被包含在紧密相连的聚类中。六倍体斯卑尔脱小麦在系统发育树中的位置介于面包小麦和硬粒小麦之间。AG基因组材料聚在一个与二倍体和其他多倍体小麦都相距较远的位置。二倍体和多倍体小麦A基因组之间的相似性估计表明，与Am相比，Au与多倍体小麦基因组的相似性高出约20%。提莫菲维小麦AG基因组在分子水平上与Au和Am小麦的基因组距离相等。
