Zhao Han, Bughrara Suleiman S, Oliveira Jose Alberto
Department of Crop and Soil Sciences, Michigan State University, East Lansing, 48823, USA.
Genome. 2006 Apr;49(4):328-35. doi: 10.1139/g05-113.
Colonial bentgrass (Agrostis capillaris L.) is a potential source for genetic improvement of resistance to environmental stress and disease for other bentgrass species (Agrostis spp.). To conserve and study the existing genetic resources of colonial bentgrass for use in breeding, genetic diversity was investigated using amplified fragment length polymorphism (AFLP) markers. Included in this study were 22 accessions from US Department of Agriculture germplasm collected from 11 countries, in conjunction with 14 accessions from northern Spain and 3 commercial cultivars. Ten EcoRI-MseI and 6 PstI-MseI AFLP primer combinations produced 181 and 128 informative polymorphic bands, respectively. Cluster analysis of genetic similarity estimates revealed a high level of diversity in colonial bentgrass species with averages of 0.51 (EcoRI-MseI) and 0.63 (PstI-MseI). Greater genetic diversity was detected by the EcoRI-MseI AFLP primer combinations. A low but significant positive correlation (r = 0.44, p = 0.0099) between the 2 Jaccard similarity matrices was obtained by the Mantel test. Commercial cultivars of bentgrass showed a narrow genetic background. The assessment of genetic diversity among colonial bentgrass accessions suggested the potential value of the colonial bentgrass germplasm in turfgrass cultivar improvement.
匍匐翦股颖（Agrostis capillaris L.）是其他翦股颖属（Agrostis spp.）物种抗环境胁迫和抗病性遗传改良的潜在资源。为了保存和研究匍匐翦股颖现有的遗传资源以用于育种，利用扩增片段长度多态性（AFLP）标记对其遗传多样性进行了研究。本研究包括从美国农业部种质库收集的来自11个国家的22份材料，以及来自西班牙北部的14份材料和3个商业品种。10对EcoRI-MseI和6对PstI-MseI AFLP引物组合分别产生了181条和128条信息性多态性条带。遗传相似性估计的聚类分析表明，匍匐翦股颖物种具有高度的多样性，EcoRI-MseI组合的平均相似性为0.51，PstI-MseI组合的平均相似性为0.63。EcoRI-MseI AFLP引物组合检测到更高的遗传多样性。通过Mantel检验，在两个Jaccard相似性矩阵之间获得了低但显著的正相关（r = 0.44，p = 0.0099）。翦股颖商业品种的遗传背景较窄。对匍匐翦股颖材料间遗传多样性的评估表明了匍匐翦股颖种质在草坪草品种改良中的潜在价值。
