Department of Plant Biology and Pathology, School of Environmental and Biological Sciences, Rutgers University, 59 Dudley Rd., Foran Hall, New Brunswick, NJ 08901-8520, USA.
Mol Ecol Resour. 2011 Sep;11(5):872-6. doi: 10.1111/j.1755-0998.2011.03006.x. Epub 2011 Mar 16.
Creeping bentgrass (Agrostis stolonifera L.) is a versatile, cross-pollinated, temperate and perennial turfgrass species. It occurs naturally in a wide variety of habitats and is also cultivated on golf courses, bowling greens and tennis courts worldwide. Isozymes and amplified fragment length polymorphisms (AFLPs) have been used to determine genetic diversity, and restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNA (RAPDs) were used to construct a genetic linkage map of this species. In the current report, we developed and characterized 215 unique genomic simple sequence repeat (SSR) markers in creeping bentgrass. The SSRs reported here are the first available markers in creeping bentgrass to date. Eight hundred and eighteen alleles were amplified by 215 SSR loci, an average of 3.72 alleles per locus. Fifty-nine per cent of those alleles segregated in a 1:1 Mendelian fashion (P > 0.05). Twenty-two per cent had a distorted segregation ratio (P ≤ 0.05). These SSR markers will be useful for assessing genetic diversity in creeping bentgrass and will be important for the development of genetic linkage maps and identifying quantitative trait loci. These markers could enhance breeding programmes by improving the efficiency of selection techniques.
匍匐翦股颖（Agrostis stolonifera L.）是一种用途广泛的、异花授粉的、温带多年生草坪草。它自然分布于多种生境中，也在全球范围内的高尔夫球场、保龄球草坪和网球场上种植。同工酶和扩增片段长度多态性（AFLPs）已被用于确定遗传多样性，而限制性片段长度多态性（RFLPs）和随机扩增多态性 DNA（RAPDs）则被用于构建该物种的遗传连锁图谱。在本报告中，我们在匍匐翦股颖中开发并鉴定了 215 个独特的基因组简单序列重复（SSR）标记。这些 SSR 是迄今为止匍匐翦股颖中第一批可用的标记。215 个 SSR 位点可扩增出 818 个等位基因，平均每个位点 3.72 个等位基因。其中 59%以 1:1 的孟德尔方式分离（P＞0.05）。22%的等位基因具有偏分离比（P≤0.05）。这些 SSR 标记将有助于评估匍匐翦股颖的遗传多样性，并将有助于遗传连锁图谱的构建和鉴定数量性状基因座。这些标记可以通过提高选择技术的效率来增强育种计划。
