Ferezou Isabelle, Bolea Sonia, Petersen Carl C H
Laboratory of Sensory Processing, Brain Mind Institute, SV-BMI-LSENS AAB 105, Station 15, Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne, Switzerland.
Neuron. 2006 May 18;50(4):617-29. doi: 10.1016/j.neuron.2006.03.043.
Voltage-sensitive dye imaging resolves the spatiotemporal dynamics of supragranular subthreshold cortical activity with millisecond temporal resolution and subcolumnar spatial resolution. We used a flexible fiber optic image bundle to visualize voltage-sensitive dye dynamics in the barrel cortex of freely moving mice while simultaneously filming whisker-related behavior to generate two movies matched frame-by-frame with a temporal resolution of up to 2 ms. Sensory responses evoked by passive whisker stimulation lasted longer and spread further across the barrel cortex in awake mice compared to anesthetized mice. Passively evoked sensory responses were large during behaviorally quiet periods and small during active whisking. However, as an exploring mouse approached an object while whisking, large-amplitude, propagating cortical sensory activity was evoked by active whisker-touch. These experiments demonstrate that fiber optics can be used to image cortical sensory activity with high resolution in freely moving animals. The results demonstrate differential processing of sensory input depending upon behavior.
电压敏感染料成像以毫秒级的时间分辨率和亚柱状空间分辨率解析了颗粒上层阈下皮质活动的时空动态。我们使用了一种灵活的光纤图像束来可视化自由活动小鼠桶状皮质中的电压敏感染料动态，同时拍摄与胡须相关的行为，以生成两部逐帧匹配的电影，时间分辨率高达2毫秒。与麻醉小鼠相比，清醒小鼠中被动触须刺激诱发的感觉反应持续时间更长，并且在桶状皮质中传播得更远。被动诱发的感觉反应在行为安静期较大，在主动动须期较小。然而，当一只探索的小鼠在动须时接近一个物体时，主动触须接触会诱发大幅度的、传播性的皮质感觉活动。这些实验表明，光纤可用于在自由活动的动物中高分辨率地成像皮质感觉活动。结果表明，感觉输入的处理因行为而异。
