Halkett Fabien, Simon Jean-Christophe, Balloux François
UMR INRA/AgroCampus de Rennes Biologie des Organismes et des Populations appliquée à la Protection des Plantes (BIO3P) INRA B.P. 35327, 35653 Le Rheu Cedex, France.
Trends Ecol Evol. 2005 Apr;20(4):194-201. doi: 10.1016/j.tree.2005.01.001. Epub 2005 Jan 12.
Many clonal organisms experience occasional events of sexual recombination, with profound consequences for their population dynamics and evolutionary trajectories. With the recent development of polymorphic genetic markers and new statistical methods, we now have an unprecedented ability to detect recombination in organisms that are thought to reproduce strictly, or essentially asexually. However, it is not always obvious which methodology to apply. Consequently, biologists might decide how to analyse their data without clear guidelines. Here, we discuss the available methods, focusing on those best suited when working with limited genetic information, such as a few genetic markers or DNA sequences. We conclude by commenting on the prospects offered by some recent conceptual advances and the access to high throughput technologies in an increasing number of model organisms.
许多克隆生物会偶尔经历有性重组事件，这对其种群动态和进化轨迹有着深远影响。随着多态性遗传标记和新统计方法的最新发展，我们现在有了前所未有的能力来检测那些被认为严格进行无性繁殖或基本无性繁殖的生物中的重组现象。然而，应用哪种方法并不总是显而易见的。因此，生物学家可能在没有明确指导方针的情况下决定如何分析他们的数据。在这里，我们讨论现有的方法，重点关注那些在处理有限遗传信息（如少数遗传标记或DNA序列）时最适用的方法。最后，我们对一些近期概念进展以及越来越多模式生物中高通量技术的应用所带来的前景进行评论。
