Kerttula Anne-Marie, Carlson Petteri, Sarkonen Nanna, Hall Val, Könönen Eija
Anaerobe Reference Laboratory, Department of Microbiology, National Public Health Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
Anaerobe. 2005 Feb-Apr;11(1-2):99-108. doi: 10.1016/j.anaerobe.2004.11.002. Epub 2005 Feb 1.
In clinical microbiology laboratories, the identification of Actinomyces-like bacteria can be very laborious and problematic. In the present study, we focused on reactivity patterns of 4 commercial test kits, RapID ANA II, RapID 32A, RapID CB Plus, and BBL Crystal ANR ID, that could be used for rapid preliminary identification of Actinomyces isolates belonging to newly described Actinomyces and closely related species. Out of the 54 strains tested, 25 strains (46%) were correctly identified to the genus/group level by BBL Crystal ANR ID system, 16 strains (30%) by RapID 32 A, 11 strains (20%) by RapID CB Plus, and 7 strains (13%) by RapID ANA II. The main problems with these kits were due to occasional weak enzymatic and sugar fermentation reactions. In conclusion, chromogenic substrate sensitivity and specificity need to be enhanced in order to improve the reliability of the test results of these kits, and the present database updated in order to more precisely identify newly described Actinomyces and closely related species.
在临床微生物学实验室中，放线菌样细菌的鉴定可能非常费力且存在问题。在本研究中，我们重点关注了4种商业测试试剂盒（RapID ANA II、RapID 32A、RapID CB Plus和BBL Crystal ANR ID）的反应模式，这些试剂盒可用于快速初步鉴定属于新描述的放线菌及密切相关物种的放线菌分离株。在所测试的54株菌株中，BBL Crystal ANR ID系统将25株（46%）正确鉴定到属/组水平，RapID 32 A鉴定出16株（30%），RapID CB Plus鉴定出11株（20%），RapID ANA II鉴定出7株（13%）。这些试剂盒的主要问题在于偶尔出现的酶促反应和糖发酵反应较弱的情况。总之，需要提高显色底物的敏感性和特异性，以提高这些试剂盒测试结果的可靠性，并更新现有数据库，以便更准确地鉴定新描述的放线菌及密切相关物种。
