Brander M A, Jousimies-Somer H R
Department in Lappeenranta, National Public Health Institute, Lappeenranta, Finland.
J Clin Microbiol. 1992 Dec;30(12):3112-6. doi: 10.1128/jcm.30.12.3112-3116.1992.
Classification and identification of fermentative actinomycetes are labor-intensive and problematic. In this study, we evaluated the applicability and reliability of the RapID ANA II system (Innovative Diagnostic Systems, Inc., Atlanta, Ga.) and the discriminatory value of the API ZYM system (Societes Analytab Products Inc., La Balme Les Grottes, France) in the identification of Actinomyces-like bacteria by using conventional methods as a reference. Eighty-five strains, including 71 isolates from mixed anaerobic infections and 14 reference strains, were tested. The RapID ANA II system correctly identified all Actinomyces odontolyticus strains and 65% of Actinomyces israelii strains. All Arcanobacterium haemolyticum strains were misidentified as Actinomyces pyogenes. The most common isolates in the study were Actinomyces meyeri-like organisms, 84% of which, however, were aerotolerant. The identification of these aerotolerant strains thus remains unresolved and warrants further studies. New characteristics and changes to the conventional API ZYM enzyme profiles are suggested. The API ZYM enzyme profiles of A. odontolyticus and A. israelii were very similar, the only discriminating enzyme being alpha-fucosidase. In differentiation between A. pyogenes and Arcanobacterium haemolyticum, the production of beta-glucuronidase by the former and the production of acid phosphatase by the latter are suggested as new helpful characteristics for use in clinical laboratories. In summary, the RapID ANA II and API ZYM systems can be used as rapid preliminary methods in the identification of Actinomyces species but accurate identification requires supplementary conventional tests and gas-liquid chromatography.
发酵放线菌的分类和鉴定工作强度大且存在问题。在本研究中，我们以传统方法为参照，评估了RapID ANA II系统（创新诊断系统公司，佐治亚州亚特兰大）的适用性和可靠性，以及API ZYM系统（法国拉巴尔姆莱格罗特的Analytab产品公司）在鉴定类放线菌属细菌方面的鉴别价值。测试了85株菌株，包括71株从混合厌氧感染中分离出的菌株和14株参考菌株。RapID ANA II系统正确鉴定出了所有溶齿放线菌菌株以及65%的以色列放线菌菌株。所有溶血隐秘杆菌菌株均被误鉴定为化脓放线菌。本研究中最常见的分离菌株是迈耶放线菌样菌，然而其中84%是耐氧菌。因此，这些耐氧菌株的鉴定仍未解决，值得进一步研究。文中提出了传统API ZYM酶谱的新特征和变化。溶齿放线菌和以色列放线菌的API ZYM酶谱非常相似，唯一具有鉴别作用的酶是α-岩藻糖苷酶。在区分化脓放线菌和溶血隐秘杆菌时，前者产生β-葡萄糖醛酸酶，后者产生酸性磷酸酶，这被建议作为临床实验室新的有用鉴别特征。总之，RapID ANA II系统和API ZYM系统可作为鉴定放线菌属物种的快速初步方法，但准确鉴定需要补充传统测试和气液色谱法。
