Hindrichsen I K, Kreuzer M, Madsen J, Bach Knudsen K E
Institute of Animal Sciences, Swiss Federal Institute of Technology Zurich (ETH), Universitaetstrasse 2, CH-8092 Zurich, Switzerland.
J Dairy Sci. 2006 Jun;89(6):2168-76. doi: 10.3168/jds.s0022-0302(06)72287-1.
Hemicelluloses, cellulose, and lignin contents of contrasting feeds, with emphasis on concentrate ingredients and complete concentrates, were analyzed using the Van Soest detergent procedure (analyzing neutral detergent fiber, acid detergent fiber, and acid detergent lignin) and the enzymatic-chemical procedure (analyzing cellulose, soluble and insoluble noncellulosic polysaccharides, and Klason lignin). Also, feces from cows fed concentrates differing in carbohydrate composition were analyzed by the 2 procedures. The correlation between acid detergent lignin and Klason lignin was significant, but not as close as the one between individual structural polysaccharides measured with the 2 procedures. The correlation between the results of the 2 procedures was highly significant for apparent cellulose digestibility, as were the correlations between digestibilities of hemicelluloses with total as well as with insoluble noncellulosic polysaccharides. The relationship between dietary lignin content and fiber digestibility was weak. The exclusion of a group of cows fed a concentrate with apple pulp, however, improved the respective correlations. Klason lignin correlated more closely with the measured fiber digestibility than acid detergent lignin. The study showed that results of the detergent method were comparable to those of the enzymatic-chemical method with cellulose, hemicelluloses, and their digestibilities. However, acid detergent lignin was much lower than Klason lignin. When the carbohydrate composition of concentrate varied widely, lignin was not suitable for the prediction of fiber digestibility.
采用范氏洗涤剂法（分析中性洗涤纤维、酸性洗涤纤维和酸性洗涤木质素）和酶 - 化学法（分析纤维素、可溶性和不可溶性非纤维素多糖以及克拉森木质素），对不同饲料（重点是精料成分和全价精料）中的半纤维素、纤维素和木质素含量进行了分析。此外，还通过这两种方法分析了饲喂碳水化合物组成不同的精料的奶牛粪便。酸性洗涤木质素和克拉森木质素之间的相关性显著，但不如用这两种方法测定的单个结构多糖之间的相关性紧密。两种方法的结果在表观纤维素消化率方面的相关性非常显著，半纤维素与总非纤维素多糖以及不可溶性非纤维素多糖消化率之间的相关性也很显著。日粮木质素含量与纤维消化率之间的关系较弱。然而，排除一组饲喂含苹果渣精料的奶牛后，相应的相关性得到了改善。克拉森木质素与测得的纤维消化率的相关性比酸性洗涤木质素更紧密。该研究表明，洗涤剂法在纤维素、半纤维素及其消化率方面的结果与酶 - 化学法相当。然而，酸性洗涤木质素远低于克拉森木质素。当精料的碳水化合物组成差异很大时，木质素不适用于预测纤维消化率。
