大肠杆菌recD突变体中λ噬菌体重组的核酸外切酶需求
Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli.
作者信息
Dermić Damir, Zahradka Davor, Petranović Mirjana
机构信息
Division of Molecular Biology, Ruder Bosković Institute, 10002 Zagreb, Croatia.
出版信息
Genetics. 2006 Aug;173(4):2399-402. doi: 10.1534/genetics.106.060426. Epub 2006 May 15.
Abstract
Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.
摘要
λ红gam噬菌体在recD突变体中的重组不受RecJ核酸外切酶失活的影响。由于核酸酶在大肠杆菌中发挥冗余作用，我们在recD突变体中使几种核酸外切酶失活，发现RecJ和核酸外切酶VII的5'-3'核酸外切酶活性对于λ重组至关重要，而3'-5'极性的核酸外切酶则是可有可无的。本文讨论了所呈现的数据对当前大肠杆菌重组起始模型的影响。
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本文引用的文献
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2
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