将大分子或小颗粒“超微量注射”到动物细胞中。一种基于病毒诱导细胞融合的新技术。
"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.
作者信息
Loyter A, Zakai N, Kulka R G
出版信息
J Cell Biol. 1975 Aug;66(2):292-304. doi: 10.1083/jcb.66.2.292.
Abstract
A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 mum) have been "injected" into cells.
摘要
本文描述了一种将大分子和小颗粒导入动物细胞的新方法。该方法的第一步是将颗粒捕获在人红细胞的空壳中。这是通过在待捕获颗粒存在的情况下使红细胞逐步溶血来实现的。第二步是仙台病毒诱导含颗粒的空壳与细胞融合。通过这种方法，已将铁蛋白和乳胶球（直径0.1微米）“注入”细胞。
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"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.将大分子或小颗粒“超微量注射”到动物细胞中。一种基于病毒诱导细胞融合的新技术。
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