完整人类红细胞与红细胞血影的融合。
Fusion of intact human erythrocytes and erythrocyte ghosts.
作者信息
Peretz H, Toister Z, Laster Y, Loyter A
出版信息
J Cell Biol. 1974 Oct;63(1):1-11. doi: 10.1083/jcb.63.1.1.
Abstract
Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepared by gradual hemolysis still retain the potential to undergo virus-induced fusion. Fusion of human red blood cells without the addition of viruses was obtained by incubation of erythrocytes at pH 10.5 in the presence of Ca(++) (40 mM) or by addition of phospholipase C Clostridium perfringens preparations to cells previously agglutinated or polylysine.
摘要
仙台病毒能够诱导人红细胞融合。二价阳离子或ATP对于多红细胞形成并非必不可少。当将仙台病毒添加到在EDTA和碘乙酸存在下孵育的细胞中时，可获得高融合指数。通过逐步溶血制备的人红细胞血影仍保留经历病毒诱导融合的潜力。通过在pH 10.5、40 mM Ca(++)存在下孵育人红细胞，或通过向先前凝集的细胞或聚赖氨酸添加产气荚膜梭菌磷脂酶C制剂，可实现不添加病毒的人红细胞融合。
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