Sekiguchi K, Asano A
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1740-4. doi: 10.1073/pnas.75.4.1740.
Fusion of washed human erythrocyte ghosts could be induced by the addition of Sendai virus after they were loaded with bovine serum albumin and resealed. Antispectrin antibody purified on a spectrin-Sepharose column and sequestered in the ghosts at 4-5 mg/ml together with the albumin was highly inhibitory for the virus-induced cell fusion, whereas Fab fragments prepared from the same antibody were without effect. The virus-induced aggregation of intramembrane particles of human erythrocytes was also inhibited by the same concentrations of the antispectrin antibody. The virus-induced agglutinations of the ghosts and release of bovine serum albumin from the ghosts (which might be caused by fusion of the viral envelope to the erythrocyte membrane) were not inhibited by the sequestered antibody. Therefore, the antibody seems to inhibit fusion at the last step--i.e., fusion between adjacent erythrocyte membranes. Similarities and differences of the mode of participation of spectrin in the virus-induced fusion and in other membrane-linked phenomena of human erythrocytes are discussed.
洗涤过的人红细胞血影在加载牛血清白蛋白并重新封闭后，加入仙台病毒可诱导其融合。在血影蛋白-琼脂糖柱上纯化并与白蛋白一起以4-5mg/ml的浓度封存于血影中的抗血影蛋白抗体对病毒诱导的细胞融合具有高度抑制作用，而由相同抗体制备的Fab片段则无此作用。相同浓度的抗血影蛋白抗体也能抑制病毒诱导的人红细胞膜内颗粒聚集。病毒诱导的血影凝集以及牛血清白蛋白从血影中的释放（这可能是由于病毒包膜与红细胞膜融合所致）不受封存抗体的抑制。因此，该抗体似乎在最后一步抑制融合，即相邻红细胞膜之间的融合。文中讨论了血影蛋白参与病毒诱导融合及人红细胞其他膜相关现象的方式的异同。
