Vaudry H, Trochard M C, Leboulenger F, Vaillant R
C R Acad Hebd Seances Acad Sci D. 1977 Mar 14;284(11):961-5.
Intermediate lobes from Rana esculenta pituitary glands were continuously superfused for 7 hrs at 28 degrees C with amphibian culture medium. alpha-MSH release was measured by use of a sensitive double antibody radio-immunoassay system. alpha-MSH secretion was inhibited by low temperatures. A large increase in alpha-MSH release was observed when Thyrotropin Releasing Hormone (TRH) at doses ranging from 10(-9) to 10(-7) molar was added to the superfusion medium. Since large amounts of TRH are to be found in the hypothalamus of amphibians but have no effect over pituitary TSH secretion, the action of TRH over alpha-MSH release may have a physiological significance.
用两栖类培养基在28℃下对食用蛙脑垂体的中间叶进行7小时的连续灌流。使用灵敏的双抗体放射免疫分析系统测定α-促黑素细胞激素（α-MSH）的释放量。低温可抑制α-MSH的分泌。当向灌流培养基中加入浓度范围为10⁻⁹至10⁻⁷摩尔的促甲状腺激素释放激素（TRH）时，观察到α-MSH释放量大幅增加。由于在两栖类动物的下丘脑可发现大量TRH，但对垂体促甲状腺激素（TSH）的分泌没有影响，因此TRH对α-MSH释放的作用可能具有生理意义。
