抗麻风砜类药物的色谱荧光分析

Chromatographic-fluorometric analysis of antileprotic sulfones.

作者信息

Murray J F, Gordon G R, Gulledge C C, Peters J H

出版信息

J Chromatogr. 1975 Apr 9;107(1):67-72. doi: 10.1016/s0021-9673(00)82748-x.

Abstract

Modifications to the power supply system of a spectrophotofluorometer are described. These modifications stabilize the output of the xenon are lamp and permit the determination of nanogram quantities of antileprotic sulfones. The sulfones are removed from plasma by a single extraction with ethyl acetate, then separated by high-pressure liquid chromatography on silica, and detected in the effluent by their fluorescence. The method is specific, rapid, and reproducible.

摘要

本文描述了对分光荧光计电源系统的改进。这些改进稳定了氙弧灯的输出，并允许测定纳克量的抗麻风砜。通过用乙酸乙酯单次萃取从血浆中去除砜，然后在硅胶上通过高压液相色谱法分离，并通过其荧光在流出物中进行检测。该方法具有特异性、快速且可重复。