Spreafico Adriano, Frediani Bruno, Capperucci Caterina, Chellini Francesca, Paffetti Alessandro, D'Ambrosio Chiara, Bernardini Giulia, Mini Roberta, Collodel Giulia, Scaloni Andrea, Marcolongo Roberto, Santucci Annalisa
Dipartimento di Medicina Clinica e Scienze Immunologiche, Policlinico Le Scotte, Università degli Studi di Siena, Italy.
Proteomics. 2006 Jun;6(12):3520-32. doi: 10.1002/pmic.200500858.
Changes in expression profiles for 17 proteins were ascertained in human mature osteoblasts compared to pre-osteoblasts (differentiation markers). A differential approach was used to highlight proteomic changes between human osteosarcoma cells and mature osteoblasts, showing a relative over-expression of 8 proteins (proliferation and tumor indicators), as well as under-expression of proteins also found down-regulated in pre-osteoblasts (specific markers of osteoblast differentiation). Our findings confirmed the differences between cell lines and primary human cell cultures and suggested caution on the use of osteosarcoma to study anti-osteoporotic drugs in humans.
与成骨前体细胞（分化标志物）相比，在人成熟成骨细胞中确定了17种蛋白质的表达谱变化。采用差异分析方法突出人骨肉瘤细胞与成熟成骨细胞之间的蛋白质组学变化，结果显示8种蛋白质（增殖和肿瘤指标）相对过表达，以及在成骨前体细胞中也被下调的蛋白质（成骨细胞分化的特异性标志物）表达不足。我们的研究结果证实了细胞系与原代人细胞培养物之间的差异，并提示在使用骨肉瘤研究人类抗骨质疏松药物时应谨慎。
