高效液相色谱法测定筋骨草中乙酰哈巴苷的含量
[Determination of acetylharpagide in Ajuga decumbens by HPLC].
作者信息
Yue Dang-Kun, Ding Guang-Zhi, Wang Wei-Hao, Wang Zhi-Min, Li Jian-Rong, Tong Yan
机构信息
Institue of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Bejing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(4):289-90.
OBJECTIVE
To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.
METHOD
The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.
RESULT
Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.
CONCLUSION
The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.
目的
建立一种定量测定筋骨草中乙酰哈巴苷的方法。
方法
色谱条件如下：采用菲罗门Luna C18柱，流动相为乙腈 - 水（15:85），流速为1.0 mL·min⁻¹，紫外吸收检测波长设定为197 nm。
结果
乙酰哈巴苷线性范围为0.6 - 3.6 μg（r = 0.9993），平均回收率和相对标准偏差分别为99.13%和2.48%。
结论
筋骨草中乙酰哈巴苷含量范围为0.40% - 6.39%。该方法简便、准确、灵敏。
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