Yang Jian-ping, Bao Bei-hua, Zhang Li, Ding An-wei
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2005 May;30(9):669-70.
To establish the determination method of pulegone in Herba Schizonepetae.
HPLC method was developed for the determination of pulegone in Herba Schizonepetae. The separation was performed on C18 column with MeOH-H2O (80:20) as a mobile phase. The detection wavelength was set at 252 nm.
The quantitation of pulegone in Herba Schizonepetae varied from 0.2-0.7 mg x g(-1). The average recovery is 96.2%, RSD 0.81%.
The method is simple, rapid and accurate.
建立荆芥中胡薄荷酮的测定方法。
采用高效液相色谱法测定荆芥中胡薄荷酮的含量。色谱柱为C18柱，以甲醇-水（80:20）为流动相，检测波长为252nm。
荆芥中胡薄荷酮的含量为0.2 - 0.7mg·g⁻¹，平均回收率为96.2%，相对标准偏差为0.81%。
该方法简便、快速、准确。
