Lin Huafeng, Li Shiguang, Zhang Lei, Wang Pingli, Zhou Yiwen
School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Ying Yong Sheng Tai Xue Bao. 2006 Feb;17(2):351-3.
With Beauveria brongniartii and Metarhizium anisopliae var. anisopliae as the reference, this paper studied the biological characteristics and invading ability of Metarhizium anisopliae var. major, a new variety separated from white grubs. The results showed that the optimal culture condition of M. anisopliae var. major was similar with that of other entomopathogenic species, and the optimal temperature was 25 degrees C. Among the three test media, PPDA was the most appropriate one, followed by SDAY and Czapek. M. anisopliae var. major had a stronger virulence than B. brongniartii at lower temperature, with an accumulative mortality of white grubs being 88.23% and 76.47%, respectively, after treated with soil and through spraying, suggesting that M. anisopliae var. major had a good prospect to widely apply in field.
以球孢白僵菌和金龟子绿僵菌变种金龟子绿僵菌为参照，本文研究了从蛴螬分离出的一个新变种大孢金龟子绿僵菌的生物学特性和侵染能力。结果表明，大孢金龟子绿僵菌的最佳培养条件与其他昆虫病原物种相似，最佳温度为25℃。在三种测试培养基中，PPDA是最合适的，其次是SDAY和察氏培养基。大孢金龟子绿僵菌在较低温度下比球孢白僵菌具有更强的毒力，经土壤处理和喷雾处理后，蛴螬的累计死亡率分别为88.23%和76.47%，表明大孢金龟子绿僵菌在田间广泛应用具有良好的前景。
