Metcalfe J W, Krogfelt K A, Krivan H C, Cohen P S, Laux D C
Department of Microbiology, University of Rhode Island, Kingston 02881.
Infect Immun. 1991 Jan;59(1):91-6. doi: 10.1128/iai.59.1.91-96.1991.
The ability of Escherichia coli K-12(K88ab) to adhere to immobilized porcine small intestine mucus was examined. E. coli K-12(K88ab) but not the isogenic E. coli K-12 strain was found to adhere readily to immobilized crude mucus but not to bovine serum albumin. The adhesion of E. coli K-12(K88ab) was inhibited in a specific fashion by anti-K88 antiserum. Adhesion was also inhibited by pretreatment of receptor-containing crude mucus preparations with sodium metaperiodate or proteolytic enzymes. Removal of glycolipids from crude mucus by chloroform-methanol extraction did not affect the ability of E. coli K-12(K88ab) to bind to mucus preparations. Adsorption of crude mucus preparations with K88ab fimbriae but not type 1 fimbriae resulted in the removal of K88-specific receptors. Analysis of the pelleted fimbriae-receptor complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, together with gel filtration chromatography of crude mucus preparations, suggest that the K88-specific receptor present in porcine small intestine mucus is a 40- to 42-kDa glycoprotein.
检测了大肠杆菌K-12（K88ab）黏附固定化猪小肠黏液的能力。发现大肠杆菌K-12（K88ab）而非同基因的大肠杆菌K-12菌株能够轻易黏附固定化的粗黏液，而不能黏附牛血清白蛋白。抗K88抗血清以特异性方式抑制了大肠杆菌K-12（K88ab）的黏附。用偏高碘酸钠或蛋白水解酶对含受体的粗黏液制剂进行预处理也会抑制黏附。通过氯仿-甲醇萃取从粗黏液中去除糖脂并不影响大肠杆菌K-12（K88ab）与黏液制剂结合的能力。用K88ab菌毛而非1型菌毛吸附粗黏液制剂导致K88特异性受体被去除。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析沉淀的菌毛-受体复合物，以及对粗黏液制剂进行凝胶过滤色谱分析，表明猪小肠黏液中存在的K88特异性受体是一种40至42 kDa的糖蛋白。
