Nakano Yoshio, Takeshita Toru, Yamashita Yoshihisa
Department of Preventive Dentistry, Faculty of Dental Science, Kyushu University, Fukuoka-shi, Japan.
Bioinformatics. 2006 Jul 15;22(14):1788-9. doi: 10.1093/bioinformatics/btl186. Epub 2006 May 18.
TRFMA provides a Web environment for analyzing T-RFLP results based on molecular weights of the fragments, rather than the numbers of nucleotides, to increase accuracy. The 16S rRNA data are saved as an XML file containing around 650 sequences (light version) and a MySQL database containing around 50 000 sequences (full version), which are connected to Web server via PHP5 and manipulated on an Internet browser.
TRFMA is freely available at http://myamagu.dent.kyushu-u.ac.jp/bioinformatics/trfma/index.html and can be downloaded from the same site.
TRFMA提供了一个网络环境，用于基于片段的分子量而非核苷酸数量来分析T-RFLP结果，以提高准确性。16S rRNA数据保存为一个包含约650个序列的XML文件（精简版）和一个包含约50000个序列的MySQL数据库（完整版），它们通过PHP5连接到网络服务器，并在互联网浏览器上进行操作。
TRFMA可从http://myamagu.dent.kyushu-u.ac.jp/bioinformatics/trfma/index.html免费获取，并可从同一网站下载。
