突触小泡蛋白跨膜结构域的构象
Conformation of the synaptobrevin transmembrane domain.
作者信息
Bowen Mark, Brunger Axel T
机构信息
The Howard Hughes Medical Institute and Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305-5489, USA.
出版信息
Proc Natl Acad Sci U S A. 2006 May 30;103(22):8378-83. doi: 10.1073/pnas.0602644103. Epub 2006 May 18.
Abstract
The synaptic vesicle protein synaptobrevin (also called VAMP, vesicle-associated membrane protein) forms part of the SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) complex, which is essential for vesicle fusion. Additionally, the synaptobrevin transmembrane domain can promote lipid mixing independently of complex formation. Here, the conformation of the transmembrane domain was studied by using circular dichroism and attenuated total reflection Fourier-transform infrared spectroscopy. The synaptobrevin transmembrane domain has an alpha-helical structure that breaks in the juxtamembrane region, leaving the cytoplasmic domain unstructured. In phospholipid bilayers, infrared dichroism data indicate that the transmembrane domain adopts a 36 degrees angle with respect to the membrane normal, similar to that reported for viral fusion peptides. A conserved aromatic/basic motif in the juxtamembrane region may be causing this relatively high insertion angle.
摘要
突触小泡蛋白突触结合蛋白（也称为VAMP，即囊泡相关膜蛋白）是可溶性N - 乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）复合体的一部分，该复合体对于囊泡融合至关重要。此外，突触结合蛋白跨膜结构域可独立于复合体形成而促进脂质混合。在此，利用圆二色光谱和衰减全反射傅里叶变换红外光谱对跨膜结构域的构象进行了研究。突触结合蛋白跨膜结构域具有α - 螺旋结构，该结构在近膜区域断裂，使得胞质结构域呈无规卷曲状态。在磷脂双分子层中，红外二色性数据表明跨膜结构域相对于膜法线呈36度角，这与报道的病毒融合肽的情况相似。近膜区域中一个保守的芳香族/碱性基序可能导致了这种相对较高的插入角度。
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