Hutchinson D W, Merigan T C
J Gen Virol. 1975 Jun;27(3):403-7. doi: 10.1099/0022-1317-27-3-403.
A local antiviral effect can be observed when (poly rI)-(poly rC), bound to Visking discs by u.v. irradiation, is incubated with monolayers of human foreskin fibroblast cells. Radioactive labelling of cytosine residues in (poly rI)-(poly rC) with -125I, has provided a much more sensitive method for determining the fate of the insoluble (poly rI)-(poly rC) than has been available hitherto. The antiviral effect is not related to the amount of (poly rI)-(POLY RC) present on the insoluble support but rather to the amount of polynucleotide lost from the support during incubation. Treatment of (poly rI)-(poly rC) which had been bound to cyanogen bromide-activated Sepharose with eigher dilute alkali or pancreatic ribonuclease released virtually all the polynucleotide. A small amount of (poly rI)-(poly rC) is released from the insoluble matrix in the presence of serum-free Minimum Eagle's Medium.
当通过紫外线照射与透析袋结合的（聚肌苷酸）-（聚胞苷酸）与人类包皮成纤维细胞单层一起孵育时，可以观察到局部抗病毒作用。用¹²⁵I对（聚肌苷酸）-（聚胞苷酸）中的胞嘧啶残基进行放射性标记，为确定不溶性（聚肌苷酸）-（聚胞苷酸）的去向提供了一种比以往任何方法都更灵敏的方法。抗病毒作用与不溶性载体上存在的（聚肌苷酸）-（聚胞苷酸）的量无关，而是与孵育过程中从载体上损失的多核苷酸量有关。用稀碱或胰核糖核酸酶处理与溴化氰活化的琼脂糖结合的（聚肌苷酸）-（聚胞苷酸），几乎可以释放出所有的多核苷酸。在无血清的伊格尔基础培养基存在下，少量的（聚肌苷酸）-（聚胞苷酸）从不溶性基质中释放出来。
