Christopoulos Th A, Papageorgakopoulou N, Theocharis D A, Mastronikolis N S, Papadas Th A, Vynios D H
Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece.
Biochim Biophys Acta. 2006 Jul;1760(7):1039-45. doi: 10.1016/j.bbagen.2006.03.019. Epub 2006 Apr 4.
Squamous cell laryngeal carcinoma undergoes significant structural-related modifications of the extracellular matrix components (ECM), the most characteristics being the presence of degraded collagen, aggrecan and hyaluronan. We examined the presence of hyaluronidase and of the cellular hyaluronan receptor CD44 during the various stages of cancer. ECM components were extracted by using PBS, 4 M GdnHCl and 4 M GdnHCl-0.1% Triton-X 100 sequentially and hyaluronidase and CD44 analyzed by zymography and immunochemistry techniques. Total RNA was also extracted and the mRNA of the various hyaluronidases and of CD44 was analyzed after amplification with RT-PCR. Hyaluronidase was detected as a double band of 45 and 55 kDa molecular mass, only in cancer samples. The analysis of mRNA indicated an aberrant expression of PH-20, the testicular-type hyaluronidase, at late stages of cancer and an overexpression of HYAL1 only at stage IV. In addition, CD44 was identified in two protein bands of 80 and 64 kDa in cancer samples. The analysis of mRNA showed that hyaluronan receptor was expressed in a stage-related order. Thus, it could be suggested that in laryngeal squamous cell carcinoma, cancer cells migrated and proliferated under the influence of small molecular mass hyaluronan, by expressing increased amounts of its receptor.
喉鳞状细胞癌会经历细胞外基质成分（ECM）显著的结构相关改变，最典型的特征是存在降解的胶原蛋白、聚集蛋白聚糖和透明质酸。我们研究了透明质酸酶和细胞透明质酸受体CD44在癌症各个阶段的存在情况。依次使用PBS、4 M盐酸胍和4 M盐酸胍 - 0.1% Triton-X 100提取ECM成分，并用酶谱法和免疫化学技术分析透明质酸酶和CD44。还提取了总RNA，并用逆转录聚合酶链反应（RT-PCR）扩增后分析各种透明质酸酶和CD44的mRNA。仅在癌症样本中检测到透明质酸酶为分子量45和55 kDa的两条带。mRNA分析表明，睾丸型透明质酸酶PH-20在癌症晚期表达异常，而HYAL1仅在IV期过度表达。此外，在癌症样本中鉴定出CD44存在于80和64 kDa的两条蛋白带中。mRNA分析表明透明质酸受体按阶段相关顺序表达。因此，可以认为在喉鳞状细胞癌中，癌细胞在小分子质量透明质酸的影响下，通过增加其受体的表达而迁移和增殖。
