鸵鸟中的雌性特异性DNA序列。

Female-specific DNA sequences in ostriches.

作者信息

Wu Chean-Ping, Horng Yan-Ming, Yang Kuo-Tai, Huang Chang-Wen, Huang Mu-Chiou

机构信息

Department of Animal Science, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, Taiwan.

出版信息

Mol Cell Probes. 2006 Oct;20(5):307-10. doi: 10.1016/j.mcp.2006.03.002. Epub 2006 Apr 4.

DOI:10.1016/j.mcp.2006.03.002

PMID:16714099

Abstract

Ostrich absence of heteromorphic sex chromosomes, unique sequences or markers located in the ostrich W-chromosome. Random amplified polymorphic DNA (RAPD) fingerprinting was carried out to investigate the sex-specific DNA sequence for sexing in ostrich. One hundred and forty random primers were used for random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). One of these primers, OPAJ-13, produced a sex-specific band only found in tested females, which was isolated and constructed into plasmids for nucleotide sequencing. A 760bp novel female-specific sequence was obtained. Two primers (OstSexOPAJ13-F and -R) were designed according to the cloned female sequence to amplify the female-specific fragment from genomic DNA of ostriches for sexing by PCR. The sex-specific band was represented in females but none were found in the males. This result showed that the sex of ostrich could be easily and effectively identified using the female-specific primers for PCR technique.

摘要

鸵鸟缺乏异形性染色体，也没有位于鸵鸟W染色体上的独特序列或标记。采用随机扩增多态性DNA（RAPD）指纹技术研究鸵鸟性别鉴定的性别特异性DNA序列。使用140个随机引物进行随机扩增多态性DNA-聚合酶链反应（RAPD-PCR）。其中一个引物OPAJ-13产生了一个仅在受试雌性中发现的性别特异性条带，将其分离并构建到质粒中进行核苷酸测序。获得了一个760bp的新的雌性特异性序列。根据克隆的雌性序列设计了两个引物（OstSexOPAJ13-F和-R），用于从鸵鸟基因组DNA中扩增雌性特异性片段，通过PCR进行性别鉴定。雌性出现了性别特异性条带，而雄性中未发现。该结果表明，使用雌性特异性引物的PCR技术可以轻松、有效地鉴定鸵鸟的性别。