Wu Chean-Ping, Horng Yan-Ming, Wang Rean-Tsz, Yang Kuo-Tai, Huang Mu-Chiou
Department of Animal Science, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, Taiwan.
Theriogenology. 2007 Jan 15;67(2):328-33. doi: 10.1016/j.theriogenology.2006.08.001. Epub 2006 Sep 11.
That most Columbidae birds have no conspicuous sexual dimorphism often makes it difficult to identify their sex on the basis of external morphology. In the present study, we report a novel sex-specific DNA marker in Columbidae birds. DNA was extracted from one member of this bird group, Streptopelia orientalis (S. orientalis, oriental turtle dove), and used to identify a female-specific DNA marker using a random amplified polymorphic DNA (RAPD) fingerprinting. One hundred and sixty random primers were used for the RAPD-PCR reactions. When using the OPAV17 primer, a novel 902 bp sex-specific PCR product was amplified from known female birds. This fragment of DNA was cloned and sequenced. Two primers, TurSexOPAV17-F and TurSexOPAV17-R, were designed from the cloned sex-specific sequence, and were successfully used to amplify a 777 bp female-specific fragment using PCR from S. orientalis DNA. This sex-specific marker was also amplified from genomic DNA samples of two other female Columbidae, S. chinensis and Columba livia. Sequence analysis showed that this novel sex-specific marker was highly conserved amongst these three bird species. In contrast, the PCR product was not amplified from male DNA of these species, nor from either sex of the S. chinensis formosa birds. Therefore, we concluded that our novel marker can be used to rapidly and accurately identify the sex of birds from three species of Columbidae.
大多数鸠鸽科鸟类没有明显的两性异形，这常常使得基于外部形态来鉴别它们的性别变得困难。在本研究中，我们报告了一种鸠鸽科鸟类新的性别特异性DNA标记。从该鸟类群体的一个成员，即东方珠颈斑鸠（Streptopelia orientalis，简称S. orientalis）中提取DNA，并使用随机扩增多态性DNA（RAPD）指纹技术来鉴定一个雌性特异性DNA标记。160种随机引物用于RAPD-PCR反应。当使用OPAV17引物时，从已知的雌性鸟类中扩增出了一个新的902 bp的性别特异性PCR产物。该DNA片段被克隆并测序。根据克隆的性别特异性序列设计了两个引物，TurSexOPAV17-F和TurSexOPAV17-R，并成功地利用PCR从东方珠颈斑鸠的DNA中扩增出一个777 bp的雌性特异性片段。这个性别特异性标记也从另外两种雌性鸠鸽科鸟类，即中华珠颈斑鸠和家鸽的基因组DNA样本中扩增出来。序列分析表明，这个新的性别特异性标记在这三种鸟类中高度保守。相反，该PCR产物在这些物种的雄性DNA中未被扩增出来，在台湾珠颈斑鸠的任何性别的DNA中也未被扩增出来。因此，我们得出结论，我们的新标记可用于快速准确地鉴定三种鸠鸽科鸟类的性别。
