Ishida Toru, Nakano Kiyoharu, Nakatani Hayao, Gomi Akihiko
Department of Cardiovascular Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
Surg Today. 2006;36(6):504-7. doi: 10.1007/s00595-006-3178-9.
Airborne bacteria in the environment are thought to be a cause of postoperative infection. With the relocation of our hospital, the operating room we had used for 35 years was replaced, changing the surgical environment for cardiac operation completely. We conducted this study to evaluate the bacteriological change in the surgical environment between the new and old operating rooms.
Airborne contaminants in the operating rooms were collected on blood agar plates, and samples of intraoperative salvaged blood from cardiac surgery were drawn from salvaged bags produced by Cell Saver 5 (Haemonetics, Braintree, MA, USA) in both the old (group O) and the new operating rooms (group N). These samples were cultured and evaluated bacteriologically.
We collected nine samples of airborne contaminants from both group O and group N. The mean number of isolated bacteria colonies was 5.0 +/- 1.2 in group O, and 2.0 +/- 0.94 in group N (P < 0.001). Bacterial growth was detected in 85% of the salvaged blood samples from group O (n = 20) versus 60% from group N (n = 15) (P = 0.09). The mean bacteria count was 1.9 +/- 2.7 colony-forming units (cfu)/ml in group O versus 0.4 +/- 0.5 cfu/ml in group N (P = 0.032).
Hospital relocation resulted in an improved operating room environment with less bacterial contamination of intraoperative salvaged blood.
环境中的空气传播细菌被认为是术后感染的一个原因。随着我院的搬迁，我们使用了35年的手术室被更换，心脏手术的手术环境彻底改变。我们进行这项研究以评估新旧手术室手术环境中的细菌学变化。
手术室中的空气传播污染物收集在血琼脂平板上，并且从旧手术室（O组）和新手术室（N组）中由Cell Saver 5（美国马萨诸塞州布伦特里市的Haemonetics公司）生产的回收袋中抽取心脏手术术中回收血样本。这些样本进行培养并进行细菌学评估。
我们从O组和N组各收集了9份空气传播污染物样本。O组分离出的细菌菌落平均数为5.0±1.2，N组为2.0±0.94（P<0.001）。O组85%的回收血样本（n = 20）检测到细菌生长，而N组为60%（n = 15）（P = 0.09）。O组的平均细菌计数为1.9±2.7菌落形成单位（cfu）/毫升，N组为0.4±0.5 cfu/毫升（P = 0.032）。
医院搬迁导致手术室环境改善，术中回收血的细菌污染减少。
