Zhang Xiao-jun, Chinkes David L, Cox Robert A, Wolfe Robert R
Metabolism Unit, Shriners Hospital for Children, University of Texas Medical Branch, Galveston, Texas 77550, USA.
J Surg Res. 2006 Sep;135(1):61-7. doi: 10.1016/j.jss.2006.03.003. Epub 2006 May 22.
Healing of a skin wound requires net protein deposition to repair the tissue defect and new epidermal cells to cover the wound. However, the true course of changes in rates of cell proliferation and protein deposition following skin injury has not been previously determined. This experiment was to measure DNA fractional synthetic rate (FSR), reflecting cell division, and protein FSR and fractional breakdown rate (FBR) in skin wound at three times after injury.
The experiment consisted of a surgery and a tracer infusion on separate days. During the surgery (day 0), a donor wound was created and indwelling catheters were inserted into the carotid artery and jugular vein under general anesthesia. On day 1, day 3, or day 7, stable isotope tracer infusion was performed in conscious rabbits to determine DNA FSR and protein FSR and FBR in the wound.
Protein FSR and FBR in the day 7 wound were 20.5 +/- 8.4 and 12.6 +/- 4.7%/day, respectively, which were greater (P < 0.01-0.05) than the corresponding values in the day 1 and day 3 wounds. Net protein deposition (FSR-FBR) in day 7 wound (7.9 +/- 6.0%/day) was greater (P < 0.05) than in day 3 wound (0.8 +/- 2.4%/day). DNA FSRs were 1.94 +/- 0.58, 2.43 +/- 0.96, 2.86 +/- 0.90%/day in the day 1, day 3 and day 7 wounds, respectively (P = 0.2).
The flow phase in the wound is characterized by increased protein synthesis rather than cell proliferation; net protein deposition in the wound is a major cause of protein requirements in severe burns.
皮肤伤口的愈合需要净蛋白质沉积以修复组织缺损，以及新的表皮细胞覆盖伤口。然而，皮肤损伤后细胞增殖率和蛋白质沉积率的真实变化过程此前尚未确定。本实验旨在测量损伤后三个时间点皮肤伤口中反映细胞分裂的DNA分数合成率（FSR）、蛋白质FSR和分数分解率（FBR）。
实验包括在不同日期进行的一次手术和一次示踪剂输注。在手术当天（第0天），制造一个供体伤口，并在全身麻醉下将留置导管插入颈动脉和颈静脉。在第1天、第3天或第7天，对清醒的兔子进行稳定同位素示踪剂输注，以确定伤口中的DNA FSR、蛋白质FSR和FBR。
第7天伤口的蛋白质FSR和FBR分别为20.5±8.4和12.6±4.7%/天，均高于（P<0.01-0.05）第1天和第3天伤口的相应值。第7天伤口的净蛋白质沉积（FSR-FBR）（7.9±6.0%/天）高于（P<0.05）第3天伤口（0.8±2.4%/天）。第1天、第3天和第7天伤口的DNA FSR分别为1.94±0.58、2.43±0.96、2.86±0.90%/天（P=0.2）。
伤口的流动期以蛋白质合成增加而非细胞增殖为特征；伤口中的净蛋白质沉积是严重烧伤患者蛋白质需求的主要原因。
