de Andrade Jaqueline Inês Alves, Ono Eduardo Akifumi, de Menezes Glauber Cruz, Brasil Elenice Martins, Roubach Rodrigo, Urbinati Elisabeth Criscuolo, Tavares-Dias Marcos, Marcon Jaydione Luiz, Affonso Elizabeth Gusmão
Aquaculture Department, INPA (Instituto Nacional de Pesquisas da Amazônia), Av. André Araújo, 2936, P.O. Box 478, Manaus, AM, 69060-001, Brazil.
Comp Biochem Physiol A Mol Integr Physiol. 2007 Apr;146(4):576-80. doi: 10.1016/j.cbpa.2006.03.017. Epub 2006 May 22.
This study evaluated the influence of diets supplemented with 500, 800, 1200 mg kg-1 of vitamin C (ascorbic acid or AA) and vitamin E (alpha-tocopherol or alpha-T) on the physiological responses of pirarucu fed for 2 months. Weight and mortality were not affected by dietary vitamin type or their concentrations. Significant increase (p<0.05) on the red blood cells count was obtained on treatments with 800 and 1200 mg AA kg-1 and on the hemoglobin concentration on treatment with 500 mg alpha-T kg-1 relatively to control. Mean corpuscular volume presented a significant decrease (p<0.05) on treatment with 800 and 1200 mg AA kg-1 when compared to control. Mean corpuscular hemoglobin concentration was significantly high (p<0.05) on treatment with 500 mg alpha-T kg-1. Only in vitamin C treatments, we noticed a significant increase (p<0.05) in the number of leucocytes relative to control. All fish in the vitamin-supplemented treatments, except 500 mg AA kg-1, had high total protein values compared to control. Fish treated with 800 or 1200 mg alpha-T kg-1 also showed increases in plasma glucose concentrations. Our results suggest that 800 and 1200 mg AA kg-1 are probably the most suitable concentrations for pirarucu diets, although high vitamin E diets are not necessary for quantitative leucocyte increases for this species.
本研究评估了在2个月的饲养期内，日粮中添加500、800、1200毫克/千克的维生素C（抗坏血酸或AA）和维生素E（α-生育酚或α-T）对巨骨舌鱼生理反应的影响。日粮中维生素的类型及其浓度对鱼的体重和死亡率没有影响。与对照组相比，日粮中添加800和1200毫克AA/千克时，红细胞计数显著增加（p<0.05）；日粮中添加500毫克α-T/千克时，血红蛋白浓度显著增加。与对照组相比，日粮中添加800和1200毫克AA/千克时，平均红细胞体积显著降低（p<0.05）。日粮中添加500毫克α-T/千克时，平均红细胞血红蛋白浓度显著升高（p<0.05）。仅在维生素C处理组中，白细胞数量相对于对照组显著增加（p<0.05）。与对照组相比，除了日粮中添加500毫克AA/千克的处理组外，所有添加维生素的处理组鱼的总蛋白值都较高。日粮中添加800或1200毫克α-T/千克的处理组鱼的血浆葡萄糖浓度也有所升高。我们的结果表明，800和1200毫克AA/千克可能是巨骨舌鱼日粮中最合适的浓度，尽管高维生素E日粮对于该物种白细胞数量的定量增加并非必需。
