Sabu Abdulhameed, Sarita Saradamma, Pandey Ashok, Bogar Barbara, Szakacs George, Soccol Carlos Ricardo
Biotechnology Division, Regional Research Laboratory (CSIR), Trivandrum, India.
Appl Biochem Biotechnol. 2002 Jul-Dec;102-103(1-6):251-60. doi: 10.1385/abab:102-103:1-6:251.
Solid-state fermentation of coconut oil cake has been carried out with Rhizopus oligosporus for the production of phytase. Phytase is used commercially in the animal feed industry to improve animal performance because there is a substantial and growing interest among swine and poultry producers in the application of phytase to improve the nutritional quality in animal feeds. Demonstrated benefits include improved feed yield ratios and reduction in the environmental costs associated with the disposal of animal wastes. We report the production of extracellular phytase by R. oligosporus under solid-state fermentation using coconut oil cake as substrate. Maximal enzyme production (14.29 U/g of dry substrate) occurred at pH 5.3, 30 degrees C, and 54.5% moisture content after 96 h of incubation. The addition of extra nutrients to the substrate resulted in inhibition of product formation. The results indicate the scope for production of phytase using coconut oil cake as solid substrate without additional nutrients.
利用少孢根霉对椰子油饼进行固态发酵以生产植酸酶。植酸酶在动物饲料工业中具有商业用途，可提高动物生产性能，因为猪和家禽养殖者对应用植酸酶改善动物饲料营养质量的兴趣日益浓厚且不断增长。已证实的益处包括提高饲料转化率以及降低与动物粪便处理相关的环境成本。我们报道了以椰子油饼为底物，少孢根霉在固态发酵条件下生产胞外植酸酶的情况。在30℃、pH 5.3、水分含量54.5%的条件下培养96小时后，酶产量达到最大值（14.29 U/g干底物）。向底物中添加额外营养物质会抑制产物形成。结果表明，无需添加额外营养物质，以椰子油饼为固体底物生产植酸酶具有可行性。
