Ha Kee-Yong, Koh In-Jun, Kirpalani Papni Arjandas, Kim Young-Yul, Cho Yun-Kyoung, Khang Gil-Son, Han Chang Whan
Department of Orthopedic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Spine (Phila Pa 1976). 2006 May 20;31(12):1309-13. doi: 10.1097/01.brs.0000219493.76081.d6.
Human intervertebral disc was obtained for the study of hypoxia inducible factor (HIF)-1alpha and apoptosis using immunohistochemical staining.
To study the expression of HIF-1alpha and apoptosis in herniated lumbar discs.
The presence of HIF-1alpha in human chondrocytes and rat intervertebral discs has been proven; however, to our knowledge, its expression in human intervertebral disc cells has not been reported. Apoptosis of the human intervertebral disc appears as a degenerative change caused by the aging of the intervertebral disc. To our knowledge, there is no reported study showing the correlation between apoptosis and HIF-1alpha in the human intervertebral disc.
There were 15 human intervertebral discs stained for HIF-1alpha immunohistochemically, and apoptosis was detected using the terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling method. On average, the patients were 32.9 years old. The intervertebral discs were divided into noncontained (9 patients) and contained (6) groups. For the control group, 5 disc samples were used.
The expression of HIF-1alpha was visualized in every case, with an average of 62.2% +/- 9.5% in the noncontained group, 30.5% +/- 3.6% in the contained group, and 11.4% +/- 9.3% in the control group. Apoptosis occurred in 74.3% +/- 7.3% of the cells in the noncontained group, 42.8% +/- 5.5% of the cells in the contained group, and 28% +/- 8.4% of the cells in the control group. HIF-1alpha and apoptosis expressions were both observed more frequently in the noncontained disc herniation group (P < 0.001). The correlation analysis between the degree of HIF-1alpha expression and apoptosis was also statistically significant.
HIF-1alpha and apoptosis physiologically occur in human beings. Their expression was the highest in the noncontained group. HIF-1alpha may play a crucial role for the survival of disc cells and resorption of the herniated disc in human intervertebral discs.
获取人类椎间盘，采用免疫组织化学染色法研究缺氧诱导因子（HIF）-1α与细胞凋亡情况。
研究HIF-1α及细胞凋亡在腰椎间盘突出症中的表达情况。
已证实在人类软骨细胞和大鼠椎间盘中存在HIF-1α；然而，据我们所知，其在人类椎间盘细胞中的表达尚未见报道。人类椎间盘的细胞凋亡表现为椎间盘老化引起的退行性改变。据我们所知，尚无研究报道人类椎间盘中细胞凋亡与HIF-1α之间的相关性。
对15个人类椎间盘进行HIF-1α免疫组织化学染色，并采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测细胞凋亡情况。患者平均年龄为32.9岁。将椎间盘分为非包容性（9例患者）和包容性（6例）组。对照组采用5个椎间盘样本。
在每个病例中均可见HIF-1α的表达，非包容性组平均为62.2%±9.5%，包容性组为30.5%±3.6%，对照组为11.4%±9.3%。非包容性组74.3%±7.3%的细胞发生凋亡，包容性组42.8%±5.5%的细胞发生凋亡，对照组28%±8.4%的细胞发生凋亡。在非包容性椎间盘突出症组中，HIF-1α和细胞凋亡的表达均更常见（P<0.001）。HIF-1α表达程度与细胞凋亡之间的相关性分析也具有统计学意义。
HIF-1α和细胞凋亡在人类体内生理性存在。它们在非包容性组中的表达最高。HIF-1α可能在人类椎间盘中对椎间盘细胞的存活及突出椎间盘的吸收起关键作用。
