Wu Wei-ping, Jiang Jian-ming, Qu Dong-bin, Wei Qing-zhu, Jiang Hui
Department of Spinal Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):1152-5.
To investigate the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and matrix metalloproteinase-2 (MMP-2) in degenerative human intervertebral disc.
Specimens of the nucleus pulposus of the degenerative discs were obtained from patients with lumbar disc herniation (experimental group), and those from young patients suffering thoracolumbal vertebral bursting fracture served as the control. The experimental group was divided into three subgroups according to the magnetic resonance imaging (MRI) results. The tissue specimens from all the groups were examined for HIF-1alpha and MMP-2 expressions using immunohistochemistry.
The tissues in the experimental group showed significantly higher expressions of HIF-1alpha and MMP-2 than those in the control group (P<0.05), and the expressions increased significantly with the severity of the degenerative changes of the intervertebral discs (P<0.05). A positive correlation was found between the expressions of the HIF-1alpha and MMP-2 in degenerative intervertebral discs.
HIF-1alpha and MMP-2 may participate in the degeneration of human intervertebral discs, in which process their expressions show a common pattern of enhancement with the progression of the condition.
研究缺氧诱导因子-1α（HIF-1α）和基质金属蛋白酶-2（MMP-2）在退变人椎间盘组织中的表达情况。
取腰椎间盘突出症患者退变椎间盘的髓核组织标本（实验组），以胸腰段椎体爆裂骨折年轻患者的髓核组织标本作为对照。实验组根据磁共振成像（MRI）结果分为三个亚组。采用免疫组织化学法检测所有组组织标本中HIF-1α和MMP-2的表达。
实验组组织中HIF-1α和MMP-2的表达明显高于对照组（P<0.05），且随着椎间盘退变程度的加重，其表达显著增加（P<0.05）。退变椎间盘中HIF-1α和MMP-2的表达呈正相关。
HIF-1α和MMP-2可能参与了人椎间盘退变过程，在此过程中它们的表达随病情进展呈现共同的增强模式。
