Hou Fu-Yun, Huang Ji, Lu Ju-Fei, Wang Zhou-Fei, Zhang Hong-Sheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
Yi Chuan Xue Bao. 2006 May;33(5):441-8. doi: 10.1016/S0379-4172(06)60071-1.
Glucose-6-phosphate dehydrogenase is a rate-limiting enzyme of pentose phosphate pathway, existing in cytosolic and plastidic compartments of higher plants. A novel gene encoding plastidic glucose-6-phosphate dehydrogenase was isolated from rice (Oryza sativa L.) and designated OsG6PDH2 in this article. Through semiquantitative RT-PCR approach it was found that OsG6PDH2 mRNA was weakly expressed in rice leaves, stems, immature spikes or flowered spikes, and a little higher in roots. However, the expression of OsG6PDH2 in rice seedlings was significantly induced by dark treatment. The complete opening reading frame (ORF) of OsG6PDH2 was inserted into pET30a (+), and expressed in Escherichia coli strain BL21 (DE3). The enzyme activity assay of transformed bacterial cells indicated that OsG6PDH2 encoding product had a typical function of glucose-6-phosphate dehydrogenase.
葡萄糖-6-磷酸脱氢酶是戊糖磷酸途径的限速酶，存在于高等植物的细胞质和质体区室中。本文从水稻（Oryza sativa L.）中分离出一个编码质体葡萄糖-6-磷酸脱氢酶的新基因，并将其命名为OsG6PDH2。通过半定量RT-PCR方法发现，OsG6PDH2 mRNA在水稻叶片、茎、未成熟穗或开花穗中表达较弱，在根中略高。然而，黑暗处理显著诱导了水稻幼苗中OsG6PDH2的表达。将OsG6PDH2的完整开放阅读框（ORF）插入pET30a（+）中，并在大肠杆菌BL21（DE3）菌株中表达。转化细菌细胞的酶活性测定表明，OsG6PDH2编码产物具有葡萄糖-6-磷酸脱氢酶的典型功能。
