Key National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, People's Republic of China.
Plant Cell Rep. 2011 Sep;30(9):1771-8. doi: 10.1007/s00299-011-1085-z. Epub 2011 Jul 13.
Tetraploid plants were produced from leaf explants of diploid Populus pseudo-simonii by treating the leaves with colchicine. Leaf explants were cultured on MS basal medium containing 1.78 μM BA and 1.08 μM NAA for 0, 6 and 12 days, and then transferred to the same MS liquid medium with colchicine at concentrations of 25, 50 and 75 μM for 1, 2 and 3 days. The highest efficiency of tetraploid induction was 14.6% by treating leaf explants that were pre-cultured for 6 days and then cultured in liquid MS with 50 μM colchicine for 3 days. Flow cytometric analysis was used to screen the tetraploids out from the regenerated plants and chromosome number counting was employed to confirm the polyploidy level. Size and frequency of leaf stomata between diploid and tetraploid plants were demonstrated to have significant differences.
通过用秋水仙素处理二倍体银腺杨叶片外植体，从叶片外植体中产生了四倍体植株。叶片外植体在含有 1.78 μM BA 和 1.08 μM NAA 的 MS 基本培养基上培养 0、6 和 12 天，然后转移到含有浓度为 25、50 和 75 μM 的秋水仙素的相同 MS 液体培养基中培养 1、2 和 3 天。通过预处理培养 6 天的叶片外植体，然后在含有 50 μM 秋水仙素的液体 MS 中培养 3 天，四倍体诱导的最高效率为 14.6%。流式细胞术分析用于从再生植株中筛选出四倍体，并通过染色体计数确认多倍体水平。二倍体和四倍体植株的叶片气孔大小和频率表现出显著差异。
