College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, South Korea.
Phytother Res. 2012 May;26(5):663-8. doi: 10.1002/ptr.3624. Epub 2011 Oct 11.
Cimicifugae rhizoma might be protective against osteoporosis. This study investigated the effects of Cimicifuga heracleifolia (CH), an Asian species of Cimicifugae rhizome, on bone loss in ovariectomized (OVX) mice. The C3H/HeN mice were divided into sham and OVX groups. The OVX mice were treated with vehicle, 17β-estradiol (E(2) ) or CH for 6 weeks. Serum calcium, phosphorus, E(2) concentration and serum alkaline phosphatase (ALP) activity were measured. Tibiae and femora were analysed using microcomputed tomography. The biomechanical property and osteoclast surface level were measured. Treatment with CH (i.p., 50 mg/kg of body weight, every other day) prevented the OVX-induced increase in body weight but did not alter the uterus weight of the OVX mice. Serum ALP levels and osteoclast surface levels in the OVX mice were reduced by treatment with CH. The CH significantly preserved trabecular bone mass, bone volume, trabecular number, trabecular thickness, structure model index and bone mineral density of proximal tibia metaphysis or distal femur metaphysis. However, grip strength, mechanical property and cortical bone architecture did not differ among the experimental groups. The results indicate that the supply of CH can prevent OVX-induced bone loss in mice.
升麻可能具有预防骨质疏松的作用。本研究探讨了亚洲升麻属植物升麻（CH）对去卵巢（OVX）小鼠骨丢失的影响。将 C3H/HeN 小鼠分为假手术和 OVX 组。OVX 小鼠用载体、17β-雌二醇（E(2) ）或 CH 处理 6 周。测量血清钙、磷、E(2)浓度和血清碱性磷酸酶（ALP）活性。使用微计算机断层扫描分析胫骨和股骨。测量生物力学特性和破骨细胞表面水平。用 CH（腹腔内注射，每次 50mg/kg 体重，每隔一天）治疗可预防 OVX 引起的体重增加，但不改变 OVX 小鼠的子宫重量。CH 治疗可降低 OVX 小鼠的血清 ALP 水平和破骨细胞表面水平。CH 可显著维持胫骨近端干骺端或股骨远端干骺端的小梁骨量、骨体积、小梁数、小梁厚度、结构模型指数和骨矿物质密度。然而，握力、力学性能和皮质骨结构在实验组之间没有差异。结果表明，CH 的供应可以预防 OVX 诱导的小鼠骨丢失。
