卤代化合物的生物修复：脱卤细菌的比较及催化剂稳定性的提高
Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability.
作者信息
Erable Benjamin, Goubet Isabelle, Lamare Sylvain, Legoy Marie-Dominique, Maugard Thierry
机构信息
Laboratoire de Biotechnologies et de Chimie Bio-organique CNRS FRE 2766, Bâtiment Marie Curie, Université de La Rochelle, Avenue Michel Crépeau 17042 La Rochelle cedex 1, France.
出版信息
Chemosphere. 2006 Nov;65(7):1146-52. doi: 10.1016/j.chemosphere.2006.04.007. Epub 2006 May 24.
Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9.0. The most effective dehalogenase activity for 1-chlorobutane conversion was detected with Rhodococcus erythropolis NCIMB13064 and Escherichia coli BL21 (DE3) (DhaA). The presence of 2-chlorobutane or propanal in the aqueous media could inhibit the 1-chlorobutane transformation.
比较了五种细菌菌株在水介质中对卤代化合物的转化情况。根据菌株不同，静息细胞脱卤酶活性的最佳温度在30至45摄氏度之间变化，而最佳pH值从8.4升至9.0。在红平红球菌NCIMB13064和大肠杆菌BL21(DE3)(DhaA)中检测到对1-氯丁烷转化最有效的脱卤酶活性。水介质中存在2-氯丁烷或丙醛会抑制1-氯丁烷的转化。
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