Pereira-Rodrigues Nazaré, Cofré Rodrigo, Zagal José H, Bedioui Fethi
Laboratoire de Pharmacologie Chimique et Génétique, UMR CNRS 8151/U INSERM 640, Ecole Nationale Supérieure de Chimie de Paris, 11, rue Pierre et Marie Curie, 75231 Paris Cedex 05, France.
Bioelectrochemistry. 2007 Jan;70(1):147-54. doi: 10.1016/j.bioelechem.2006.03.025. Epub 2006 Apr 5.
Modified electrodes coated by adsorbed cobalt phthalocyanines are known to show substantial electrocatalytic activity for the electro-oxidation of several thiols in alkaline aqueous solution. In this context, we explore in this study the electrocatalytic activity of adsorbed cobalt phthalocyanine (CoPc) on ordinary pyrolytic graphite electrode for the oxidation of reduced L-glutathione GSH and the reduction of its disulfide GSSG at physiological pH. To do so, cyclic and rotating disk voltammetries were performed and the amperometric results show that a stable electrochemical sensing material, with good reproducibility and sensitivity (in accordance with the concentrations of GSH expected in biological media), can be easily achieved. This opens the way for the design of an electrochemical sensor able to detect these two analytes in biologically relevant experimental conditions (in terms of pH).
已知吸附有钴酞菁的修饰电极对碱性水溶液中几种硫醇的电氧化具有显著的电催化活性。在此背景下，我们在本研究中探究了吸附在普通热解石墨电极上的钴酞菁（CoPc）在生理pH值下对还原型L-谷胱甘肽（GSH）的氧化及其二硫键（GSSG）还原的电催化活性。为此，进行了循环伏安法和旋转圆盘伏安法，安培法结果表明，可以轻松实现一种具有良好重现性和灵敏度（与生物介质中预期的GSH浓度一致）的稳定电化学传感材料。这为设计一种能够在生物学相关实验条件（就pH而言）下检测这两种分析物的电化学传感器开辟了道路。
