Clerici M, Landay A L, Kessler H A, Zajac R A, Boswell R N, Muluk S C, Shearer G M
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md 20892.
J Immunol. 1991 Apr 1;146(7):2207-13.
The APC/stimulating cell (APC/SC) potential of PBMC from Walter Reed stage 1 and 2 patients and patients with AIDS was tested by using these PBMC as stimulators in an allogeneic MLR. The responding cells were PBMC from unrelated, HIV- donors that were either unfractionated or depleted of APC by plastic and nylon wool adherence. Using this approach, we observed no defect in the APC/SC potential of PBMC from Walter Reed stage 1 and 2 patients. However, PBMC from AIDS patients used as allogeneic stimulators exhibited three different patterns of APC/SC function: 1) no defect in alloantigen (ALLO) APC/SC potential; 2) a defect in ALLO APC/SC function that was detected only if the responder cells were depleted of APC (presenting cell defect); and 3) a defect in ALLO APC/SC function, irrespective of whether the responder cells were depleted of APC (stimulating cell defect). These results indicate that in addition to Th cell defects associated with AIDS, the PBMC from AIDS patients can also exhibit a defect in APC/SC function. This study provides an approach that permits the testing of Ag-presenting function in all AIDS patients, and is therefore not limited to testing patients for whom HIV-, HLA-identical T cells and APC are available.
通过在同种异体混合淋巴细胞反应（MLR）中使用来自沃尔特里德陆军医疗中心1期和2期患者以及艾滋病患者的外周血单核细胞（PBMC）作为刺激细胞，来检测其抗原呈递细胞/刺激细胞（APC/SC）潜能。反应细胞是来自不相关的、HIV阴性供体的PBMC，这些PBMC未进行分离或通过塑料和尼龙毛黏附法去除了APC。采用这种方法，我们观察到来自沃尔特里德陆军医疗中心1期和2期患者的PBMC在APC/SC潜能方面没有缺陷。然而，用作同种异体刺激细胞的艾滋病患者的PBMC表现出三种不同的APC/SC功能模式：1）同种异体抗原（ALLO）APC/SC潜能无缺陷；2）仅当反应细胞去除了APC时才检测到的ALLO APC/SC功能缺陷（呈递细胞缺陷）；3）ALLO APC/SC功能缺陷，无论反应细胞是否去除了APC（刺激细胞缺陷）。这些结果表明，除了与艾滋病相关的Th细胞缺陷外，艾滋病患者的PBMC在APC/SC功能方面也可能存在缺陷。本研究提供了一种方法，可用于检测所有艾滋病患者的抗原呈递功能，因此不限于检测有HIV阴性、HLA相同的T细胞和APC可用的患者。
