Jin Yumi, DeVries Arthur L
Department of Molecular and Integrative Biology, University of Illinois, Urbana, 61801, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2006 Jul;144(3):290-300. doi: 10.1016/j.cbpb.2006.03.006. Epub 2006 May 24.
A quantification method was developed to determine the concentrations of the major antifreeze glycoprotein (AFGP) isoforms in the blood of Antarctic notothenioid fishes. Serum samples were precipitated with 2.5% TCA and the supernatant containing AFGPs were chromatographed on an HPLC size exclusion column and the concentrations of the major AFGP size classes were determined from the areas of the corresponding peaks in the elution profile. Eight species of Antarctic notothenioid fishes were examined and their blood AFGP concentrations varied from 5 to 35 mg/mL. All of these fishes synthesized both the large and small AFGPs, but maintained higher levels of small AFGPs than the large ones in their blood. The species inhabiting more severe water environments (lower temperature and presence of ice) had higher serum AFGP levels than those in milder environments. The cryopelagic Pagothenia borchgrevinki decreased their blood AFGP concentrations in response to warm acclimation, but to a much lower extent in comparison to the antifreeze-bearing fishes in the Northern Hemisphere. After being warm acclimated at +4 degrees C for 16 weeks, the serum concentrations of the small and large AFGPs were decreased by about 60% and 20%, respectively.
开发了一种定量方法来测定南极南极鱼科鱼类血液中主要抗冻糖蛋白（AFGP）同工型的浓度。血清样本用2.5%三氯乙酸沉淀，含有AFGPs的上清液在高效液相色谱尺寸排阻柱上进行色谱分析，主要AFGP尺寸类别的浓度根据洗脱图谱中相应峰的面积来确定。对8种南极南极鱼科鱼类进行了检测，它们血液中的AFGP浓度在5至35毫克/毫升之间。所有这些鱼类都能合成大、小两种AFGPs，但血液中小AFGPs的水平高于大AFGPs。生活在更恶劣水环境（更低温度和有冰存在）中的物种，其血清AFGP水平高于生活在较温和环境中的物种。栖居于低温水域的南极红点鲑在温暖驯化后血液中的AFGP浓度降低，但与北半球的抗冻鱼类相比，降低幅度要小得多。在4℃下温暖驯化16周后，小AFGP和大AFGP的血清浓度分别降低了约60%和20%。
