Woods G L, Ginther O J
Department of Veterinary Science University of Wisconsin-Madison Madison, WI 53706 USA.
Theriogenology. 1984 Mar;21(3):461-9. doi: 10.1016/0093-691x(84)90408-4.
A pituitary extract was used to induce multiple ovulations in mares to determine whether day-7 embryos from multiple ovulators were viable as indicated by their ability to develop when transferred to recipients. There were more ovulations/donor for induced multiple-ovulating mares than for control single-ovulating mares (4.6 +/- 0.5 vs 1.0 +/- 0.0; n=14). The embryo collection rate per ovulation was similar for multiple ovulators (0.6 +/- 0.1 embryos/ovulation) and single ovulators (0.7 +/- 0.1). The embryo collection rate per donor, therefore, was higher (P<0.01) for the multiple ovulators (2.9 +/- 0.7 vs 0.7 +/- 0.1). The transfer success rate per embryo at day 21 was different (P<0.05) among recipients which received an embryo from control single-ovulating donors (7 8 ), multiple ovulators from which a single embryo was recovered (2 2 ), and multiple ovulators from which multiple embryos were recovered (9 19 ). The recipient pregnancy rate/donor at day 21 was 88% (7 8 ) for single-ovulating controls and 138% (11 8 ) for induced multiple ovulators. Results indicate that the survivability of day-7 embryos from multiple-ovulating donors was reduced. However, despite the reduced survival rate/embryo, the number of pregnant recipients/donor was increased by induction of multiple ovulations because of the increased number of embryos available for transfer.
使用垂体提取物诱导母马多次排卵，以确定多排卵母马第7天的胚胎在移植给受体后能否如预期那样发育从而判断其是否具有活力。诱导多排卵的母马每个供体的排卵数比对照单排卵母马更多（4.6±0.5对1.0±0.0；n = 14）。多排卵母马和单排卵母马每次排卵的胚胎采集率相似（分别为0.6±0.1个胚胎/次排卵和0.7±0.1个胚胎/次排卵）。因此，多排卵母马每个供体的胚胎采集率更高（P<0.01）（2.9±0.7对0.7±0.1）。在第21天，接受来自对照单排卵供体胚胎的受体、回收单个胚胎的多排卵母马来源胚胎的受体以及回收多个胚胎的多排卵母马来源胚胎的受体，每个胚胎的移植成功率有所不同（P<0.05）（分别为7/8、2/2和9/19）。第21天，单排卵对照供体每个供体的受体妊娠率为88%（7/8），诱导多排卵供体每个供体的受体妊娠率为138%（11/8）。结果表明，多排卵供体第7天胚胎的存活率降低。然而，尽管每个胚胎的存活率降低，但由于可用于移植的胚胎数量增加，通过诱导多排卵增加了每个供体的妊娠受体数量。
