Sirois J, Betteridge K J, Brault A
Can Vet J. 1987 Dec;28(12):750-3.
Embryo transfer was used in an equestrian teaching center in order to produce as many foals as possible from their preferred mares during a single breeding season. Embryo collection by uterine lavage was attempted in five donor mares on 25 occasions 6.5 days after ovulation. Sixteen of the collection attempts (64%) yielded a total of 17 blastocysts. Of these 17 embryos, 13 were immediately transferred transcervically into recipient mares that had ovulated within two days of the time of ovulation in the donors, three were frozen for later transfer, and one was lost. Eight of the freshly transferred embryos (61%) developed and were detected on ultrasonography on day 11.5; five of these continued to develop normally and gave rise to healthy foals (38%), but three were lost at 14.5, 22.5 and 24.5 days gestation. Two of the frozen embryos were judged viable when thawed the following year and produced one additional pregnancy after transcervical transfer. Thus the five donor mares have produced five foals and a sixth 90-day pregnancy(1) with only a three-month interruption of their use for competition and teaching.(1)While this paper was in press, the sixth pregnancy also terminated in the production of a healthy foal.
在一个马术教学中心采用胚胎移植技术，以便在单个繁殖季节从其优选的母马中尽可能多地繁育出幼驹。在排卵后6.5天，对5头供体母马进行了25次子宫冲洗采集胚胎的尝试。其中16次采集尝试（64%）共获得了17个囊胚。在这17个胚胎中，13个立即经宫颈移植到在供体排卵后两天内排卵的受体母马体内，3个冷冻以备后续移植，1个丢失。8个新鲜移植的胚胎（61%）发育并在第11.5天通过超声检查检测到；其中5个继续正常发育并生出健康幼驹（38%），但3个在妊娠14.5、22.5和24.5天时丢失。次年解冻时，2个冷冻胚胎被判定为存活，并在经宫颈移植后又产生了一次妊娠。因此，这5头供体母马仅在用于比赛和教学的三个月中断期内就产出了5头幼驹和第六次90天的妊娠。（1）在本文付印时，第六次妊娠也产下了一头健康幼驹。
