Kasiraj R, Misra A K, Mutha Rao M, Jaiswal R S, Rangareddi N S
Buffalo Breeding Centre, Nekarikallu - 522 615 (A.P.), India.
Theriogenology. 1993 May;39(5):1187-92. doi: 10.1016/0093-691x(93)90016-x.
A total of 141 embryos was recovered by nonsurgical flushing of the uterus of 31 superovulated buffalo. A total of 66 good quality embryos (Grade I and Grade II) was frozen using 1.4 M glycerol. Forty-two of the frozen embryos were thawed randomly over a 1-year period, and a total of 39 embryos (Grades I, II or III post thaw) were transferred into an equal number of estrus synchronized recipients. Of 11 confirmed pregnancies, 9 calves were born live.
通过对31头超排水牛的子宫进行非手术冲洗，共回收了141枚胚胎。使用1.4M甘油冷冻了66枚优质胚胎（I级和II级）。在1年的时间里随机解冻了42枚冷冻胚胎，将总共39枚胚胎（解冻后I级、II级或III级）移植到相同数量的同期发情受体中。在11例确诊妊娠中，有9头小牛存活出生。
