Garcia A, Salaheddine M
Pharming B.V., Polsbroek, The Netherlands.
Theriogenology. 1997 Apr 1;47(5):1003-8. doi: 10.1016/s0093-691x(97)00057-5.
Recent interest and research activities in ultrasound-guided transvaginal amniocentesis have contributed greatly to the development of a reliable technique, particularly with respect to a reduction in fetal loss after the puncture procedure. Several investigators have reported a variety of techniques for the collection of bovine fetal fluids at different stages of pregnancy; however, thus far, a high incidence of fetal loss has occurred as a consequence of the aspiration procedure. This manuscript describes the technical aspects of a safe and reliable ultrasound-guided transvaginal method of amniotic fluid collection from heifers (n = 93) with Day 79 to 90 bovine conceptuses using a very fine aspiration needle. Using this approach, collection of amniotic fluid samples was successful in 88 out of 93 (94.6 %) attempts, and no complications or pregnancy losses were recorded as a consequence of the procedure. The results reported here suggest that the incidence of spontaneous fetal loss associated with this transvaginal amniocentesis procedure is very satisfactory and could be the method of choice for the collection of fetal fluids in embryo transfer programs.
近期对超声引导下经阴道羊膜腔穿刺术的关注和研究活动极大地推动了一项可靠技术的发展，尤其是在降低穿刺术后胎儿丢失方面。几位研究者报告了在妊娠不同阶段采集牛胎儿液体的多种技术；然而，迄今为止，由于抽吸操作导致胎儿丢失的发生率很高。本手稿描述了一种安全可靠的超声引导经阴道方法，该方法使用非常细的抽吸针从妊娠79至90天的小母牛（n = 93）中采集羊水。采用这种方法，93次尝试中有88次（94.6%）成功采集到羊水样本，且该操作未记录到任何并发症或妊娠丢失。此处报告的结果表明，与这种经阴道羊膜腔穿刺术相关的自然胎儿丢失发生率非常令人满意，可能是胚胎移植项目中采集胎儿液体的首选方法。
