Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
Vet Rec. 2021 Apr;188(7):e31. doi: 10.1002/vetr.31. Epub 2021 Jan 19.
In humans, analysis of amniotic fluid is widely used for diagnostic and prognostic purposes. Amniocentesis has scarcely been used in veterinary medicine to date, despite a tremendous potential for clinical and research applications in dogs. Our study aimed to establish a safe method for foetal fluid sampling in female dogs.
Two transabdominal ultrasound-guided methods were assessed: the "free hand" and the needle-guided bracket sampling. In addition, through a subsequent routinely scheduled ovariohysterectomy, fluid was directly collected. Samples from 98 conceptuses were collected at day 46.7 ± 7.5 of pregnancy.
The amount of fluid retrieved varied between 0.5 and 5.0 ml per collection. Macroscopic examination of the uterus and conceptuses identified 53% of the puncture sites. Neither fluid leakage nor foetal injury was detected, and six hematomas (5.8%) were visible. Ultrasound-guided foetal fluid collection was found to be potentially safe, and it can be performed by using either transabdominal method.
Foetal fluid collection is possible with relative ease and low short-term risk, and may open paths for diagnostic, therapeutic and research purposes in dogs. The procedure can provide new insights into prenatal clinical medicine, including diagnostics of foetal deaths, early identification of heritable diseases and so on.
在人类中，羊水分析广泛用于诊断和预后目的。尽管在犬类的临床和研究应用方面具有巨大潜力，但羊膜穿刺术在兽医医学中至今鲜有应用。我们的研究旨在为母犬的胎儿液采样建立一种安全的方法。
评估了两种经腹超声引导的方法：“徒手”和针引导支架采样。此外，通过随后的常规卵巢子宫切除术，直接收集液体。在妊娠第 46.7±7.5 天采集了 98 个胚胎的样本。
每次采集的液体量在 0.5 至 5.0 毫升之间不等。对子宫和胚胎的肉眼检查确定了 53%的穿刺部位。未发现液体泄漏或胎儿损伤，有 6 个血肿（5.8%）可见。超声引导的胎儿液采集具有潜在的安全性，并且可以使用两种经腹方法之一进行。
胎儿液采集相对容易且短期风险低，这可能为犬类的诊断、治疗和研究目的开辟新途径。该程序可以为产前临床医学提供新的见解，包括胎儿死亡的诊断、遗传性疾病的早期识别等。
