Hirai M, Cerbito W A, Wijayagunawardane M P, Braun J, Leidl W, Ohosaki K, Matsuzawa T, Miyazawa K, Sato K
Obihiro University of Agriculture and Veterinary Medicine Faculty of Veterinary Medicine, Laboratory of Theriogenology Inada-cho, Obihiro-City, Hokkaido, Japan.
Theriogenology. 1997 May;47(7):1463-78. doi: 10.1016/s0093-691x(97)00136-2.
The effect of egg yolk extender on semen viscosity and bull sperm motility of fresh and cooled or deep frozen semen was determined by a computer-assisted system. Viscosity of the extender was determined by flow time. Based on the sperm velocity (velocity of the average path), individual spermatozoon were classified into groups of progressively motile (>==30 microm/sec) and immotile (<10 microm/sec) spermatozoa. The average velocity of progressively motile spermatozoa (VPM), the velocity of linear progressively motile spermatozoa (VLP) and the percentage of linear swimming spermatozoa (LIN) were evaluated. The addition of 10, 20 or 30% egg yolk to Tris buffer (pH 6.5) resulted in a linear decrease of VPM and a decrease in the percentage of progressively motile spermatozoa, but it increased the relative rate of LIN in fresh diluted semen. Increasing the levels of egg yolk in the diluent resulted in higher viscosity. The VLP was significantly higher than the VPM. In refrigerated or frozen semen samples, extender with 30 and 20% egg yolk had a similar effect on the VPM but not on the percentage of progressively motile sperm cells. Freezing of egg yolk (30%) extender to -20 degrees C resulted in a significant increased flow time and higher viscosity. Dilution of semen samples with high viscosity extender decreased the VPM in fresh and chilled semen. Freezing semen of high viscosity extender with glycerol had no apparent effect on the percentage of progressively motile spermatozoa compared with that of non-glycerinated egg yolk extender. The results suggest that different concentrations of egg yolk in the extender can influence the parameters of semen viscosity and sperm motility evaluated by a computer-assisted system.
采用计算机辅助系统测定了蛋黄稀释液对新鲜、冷藏或冷冻精液的精液粘度和公牛精子活力的影响。通过流动时间测定稀释液的粘度。根据精子速度（平均路径速度），将单个精子分为前进运动精子（≥30微米/秒）和不运动精子（<10微米/秒）组。评估前进运动精子的平均速度（VPM）、直线前进运动精子的速度（VLP）和直线游动精子的百分比（LIN）。在Tris缓冲液（pH 6.5）中添加10%、20%或30%的蛋黄会导致VPM呈线性下降，前进运动精子的百分比降低，但会增加新鲜稀释精液中LIN的相对比例。稀释液中蛋黄水平的增加会导致粘度升高。VLP显著高于VPM。在冷藏或冷冻精液样本中，含30%和20%蛋黄的稀释液对VPM有类似影响，但对前进运动精子细胞的百分比没有影响。将含30%蛋黄的稀释液冷冻至-20℃会导致流动时间显著增加和粘度升高。用高粘度稀释液稀释精液样本会降低新鲜和冷藏精液中的VPM。与未添加甘油的蛋黄稀释液相比，用甘油冷冻高粘度稀释液的精液对前进运动精子的百分比没有明显影响。结果表明，稀释液中不同浓度的蛋黄会影响通过计算机辅助系统评估的精液粘度和精子活力参数。
