Bandeira Anderson Marques Pinto, Matos José Eduardo, Maria Alexandre Nízio, Carneiro Paulo César Falanghe, Purdy Phillip H, Azevedo Hymerson Costa
Universidade Federal de Sergipe, São Cristóvão, .
Embrapa Tabuleiros Costeiros, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), , .
Anim Reprod. 2018 Aug 16;15(1):23-28. doi: 10.21451/1984-3143-AR2017-0021.
The physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH - 5.96 5.71 at 48 h and 6.13 5.89 at 72 h; PS - 21.66 78.34 at 48 h and 20.87 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits.
明胶的物理和化学特性被用来证明将其添加到精液稀释液中以保存精子质量并提高宫颈人工授精（使用冷藏精液）效果的合理性。本研究的目的是评估在冷却稀释液中添加明胶对5°C储存的公羊精液质量和生育力的影响。将圣伊内斯公羊（n = 6）的精液样本（n = 24）在不含明胶（对照组）或含1.5%明胶的甘氨酸 - 蛋黄 - 牛奶稀释液中进行稀释。样本被装入0.25 mL细管中，冷却至5°C并垂直储存48小时和72小时。使用基于pH、渗透压以及细管上下段精子比例（PS）的细管均匀性测试、精子活力分析、质膜和顶体膜完整性分析以及人工授精后的生育力评估样本质量。发现细管的上段和下段在pH和PS（%）方面存在差异。仅在两个时间点的对照组中差异显著：48小时时pH为5.96对5.71，72小时时为6.13对5.89；48小时时PS为21.66对78.34，72小时时为20.87对79.13。在含明胶的稀释液中储存对精子动力学或精子膜完整性评估几乎没有影响。向稀释液中添加明胶不影响4.4%至26.1%的妊娠率。我们得出结论，明胶在维持精液样本的物理和化学均匀性方面是有效的。需要进一步研究以优化明胶添加的使用并阐明任何潜在益处。
