Takedomi T, Kaneko H, Aoyagi Y, Konishi M, Kishi H, Watanabe G, Taya K
Central Research Institute for F and L Zen-noh, Tsukuba, Ibaraki 300-33, Japan.
Theriogenology. 1997 Jun;47(8):1507-18. doi: 10.1016/s0093-691x(97)00157-x.
The effects of acute neutralization of endogenous inhibin on ovulation rate and circulating FSH levels were investigated. Nine or ten days after estrus, 5 heifers were given a single injection of 75 ml iv inhibin antiserum produced in a castrated male goat, while another 5 were given the same amount of a castrated male goat serum. All heifers were given injections of PGF2alpha im at 48 h and 60 h after the serum injection. Those exhibiting an estrus were artificially inseminated with frozen-thawed semen. Seven or eight days after the insemination, ova or embryos were collected using a non-surgical method. Administration of inhibin antiserum resulted in a significant increase in the number of medium-sized follicles compared with the number in the control animals. The number of large follicles in the inhibin-neutralized animals was 4.8 +/- 2.4 (mean +/- SEM; n = 5) on the day of estrus, while there was a single large follicles in the ovaries of control animals. Seven or eight days after estrus, 3 to 16 ova or embryos were recovered from 4 of 5 animals, and 64 % of the total ova/embryos were transferable. Administration of inhibin antiserum produced a significant increase in the concentrations of plasma FSH from 12 to 72 h after the serum injection compared with the levels in the control animals (P < 0.05). After the onset of estrus, preovulatory LH and FSH surges were noted in inhibin-neutralized animals and magnitude of the rise in each hormone was similar to the control animals. The present study demonstrates that a single injection of the inhibin antiserum induces multiple ovulations probably by enhancing FSH secretion, and that recovery of embryos is equal to that observation after an ordinary FSH treatment.
研究了内源性抑制素急性中和对排卵率和循环促卵泡素（FSH）水平的影响。发情后9或10天，给5头小母牛静脉注射75毫升由去势雄山羊产生的抑制素抗血清，而另外5头注射等量的去势雄山羊血清。所有小母牛在血清注射后48小时和60小时肌肉注射前列腺素F2α（PGF2α）。出现发情的小母牛用冻融精液进行人工授精。授精后7或8天，采用非手术方法收集卵子或胚胎。与对照动物相比，注射抑制素抗血清导致中等大小卵泡数量显著增加。在发情当天，抑制素中和组动物的大卵泡数量为4.8±2.4（平均值±标准误；n = 5），而对照动物卵巢中只有单个大卵泡。发情后7或8天，5头动物中有4头回收了3至16个卵子或胚胎，且总卵子/胚胎的64%可用于移植。与对照动物相比，注射抑制素抗血清后12至72小时血浆FSH浓度显著升高（P < 0.05）。发情开始后，在抑制素中和组动物中观察到排卵前促黄体素（LH）和FSH高峰，且每种激素升高的幅度与对照动物相似。本研究表明，单次注射抑制素抗血清可能通过增强FSH分泌诱导多次排卵，且胚胎回收率与普通FSH处理后的观察结果相当。
