Komatsu Kouji, Masubuchi Satoru
Department of Physiology Aichi Medical University Aichi Japan.
Reprod Med Biol. 2016 Dec 26;16(1):21-27. doi: 10.1002/rmb2.12010. eCollection 2017 Jan.
The number of ovulated oocytes is different among mammals but does not vary much within the same species. In order to sustain periodic ovulation, follicular development must be coordinated at the tissue level. Elucidating the regulatory mechanisms of follicular development is difficult because the ovary has a complicated structure and it takes a long time for primordial follicles to develop into Graafian follicles. Therefore, it is not possible to observe follicular development by conventional experiments. The authors previously developed a new ovarian tissue culture method that enabled the observation of follicular development from the early follicle stage. These findings indicated that follicular interactions are important in regulating follicular development and ovulation. This review describes the current methods of observing follicular development in the ovary and the regulatory mechanisms of follicular development.
不同哺乳动物排卵的卵母细胞数量不同，但同一物种内差异不大。为了维持周期性排卵，卵泡发育必须在组织水平上进行协调。由于卵巢结构复杂，原始卵泡发育成格拉夫卵泡需要很长时间，因此阐明卵泡发育的调控机制很困难。因此，通过传统实验无法观察卵泡发育。作者此前开发了一种新的卵巢组织培养方法，能够观察从早期卵泡阶段开始的卵泡发育。这些发现表明，卵泡间相互作用在调节卵泡发育和排卵中很重要。本综述描述了目前观察卵巢卵泡发育的方法以及卵泡发育的调控机制。
